PHF19 Polyclonal antibody

• Featured Product
• KD/KO Validated

PHF19 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 11895-1-AP

Synonyms

Polycomb-like protein 3, Polycomb like protein 3, PHD finger protein 19, PCL3, MTF2L1

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Validation Data Gallery

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  WB analysis of rat brain using 11895-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11895-1-AP (PHF19 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of Mouse ES cells using 11895-1-AP

  IF result of Mouse ES cell From Kevin Corbit.

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Tested Applications

• Positive WB detected in: rat brain tissue
• Positive IF/ICC detected in: Mouse ES cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Product Information

11895-1-AP targets PHF19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHF19 fusion protein Ag2511
• Full Name: PHD finger protein 19
• Calculated Molecular Weight: 580 aa, 63 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC022374
• Gene Symbol: PHF19
• Gene ID (NCBI): 26147
• RRID: AB_10804890
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHD FINGER PROTEIN 19(PHF19), a protein homologous to the Drosophila 'Polycomblike' (Pcl) protein, encoded by human gene PCL, which encodes 2 deduced proteins: a short form of 207 aa and a long form of 580 aa. The PCL family proteins contains a PCL homology domain, a Tudor domain, 2 PHD fingers and a C-terminal tail. It is highly expressed in thymus and heart, while 508aa form predominant in placenta, skeletal muscle and kidney and 207aa form in liver and peripheral blood leukocytes. Both of these forms can inhibit transcriptional activity. And the long form is corelated with cancer.Acts as a transcritpional repressor. The mRNA level of PHF19 is low in many normal tissue, it's elevated in cancer-derived cell lines.

Publications

Species	Application	Title
mouse	WB	Mol Cell; An H3K36 methylation-engaging Tudor motif of polycomb-like proteins mediates PRC2 complex targeting.; Authors - Ling Cai ; KD Validated
human		PLoS Genet; Alu Elements in ANRIL Non-Coding RNA at Chromosome 9p21 Modulate Atherogenic Cell Functions through Trans-Regulation of Gene Networks.; Authors - Holdt Lesca M LM
mouse	WB	PLoS Genet; Polycomb-like 3 promotes polycomb repressive complex 2 binding to CpG islands and embryonic stem cell self-renewal.; Authors - Julie Hunkapiller ; KD Validated
human	IHC	Front Immunol; Comprehensive Pan-Cancer Genomic Analysis Reveals PHF19 as a Carcinogenic Indicator Related to Immune Infiltration and Prognosis of Hepatocellular Carcinoma.; Authors - Zheng-Yi Zhu
human,mouse	WB	J Cancer; ZNF750 exerted its Antitumor Action in Oral Squamous Cell Carcinoma by regulating E2F2.; Authors - Hong-Li Yang
human	WB,IHC	Scand J Gastroenterol; Downregulation of PHF19 inhibits cell growth and migration in gastric cancer.; Authors - Haixiao Wang ; KD Validated