Tested Applications
| Positive WB detected in | rat brain tissue |
| Positive IF/ICC detected in | Mouse ES cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11895-1-AP targets PHF19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2511 Product name: Recombinant human PHF19 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-207 aa of BC022374 Sequence: MENRALDPGTRDSYGATSHLPNKGALAKVKNNFKDLMSKLTEGQYVLCRWTDGLYYLGKIKRVSSSKQSCLVTFEDNSKYWVLWKDIQHAGVPGEEPKCNICLGKTSGPLNEILICGKCGLGYHQQCHIPIAGSADQPLLTPWFCRRCIFALAVRVSLPSSPVPASPASSSGADQRLPSQSLSSKQKGHTWALETDSASATVLGQDL Predict reactive species |
| Full Name | PHD finger protein 19 |
| Calculated Molecular Weight | 580 aa, 63 kDa |
| Observed Molecular Weight | 22 kDa, 66 kDa |
| GenBank Accession Number | BC022374 |
| Gene Symbol | PHF19 |
| Gene ID (NCBI) | 26147 |
| RRID | AB_10804890 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T6S3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PHD FINGER PROTEIN 19(PHF19), a protein homologous to the Drosophila 'Polycomblike' (Pcl) protein, encoded by human gene PCL, which encodes 2 deduced proteins: a short form of 207 aa and a long form of 580 aa. The PCL family proteins contains a PCL homology domain, a Tudor domain, 2 PHD fingers and a C-terminal tail. Both of these forms can inhibit transcriptional activity. And the long form is corelated with cancer. The mRNA level of PHF19 is low in many normal tissue, it's elevated in cancer-derived cell lines.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PHF19 antibody 11895-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PHF19 antibody (Cat# 11895-1-AP) has 13 total citations. Journals include Nature Communications, Molecular Cell, PLoS Genetics, Frontiers in Immunology, Brain Research Bulletin, Pathology, Journal of Proteome Research, Journal of Cancer, Scandinavian Journal of Gastroenterology, Stem Cell Research, and Oncotarget. Research fields span epigenetics and chromatin biology (PRC2, histone modifications), oncology (hepatocellular carcinoma, gastric cancer, prostate cancer, lymphoma, esophageal and oral cancers), Alzheimer's disease, atherosclerosis, and stem cell biology.
| Species | Application | Title |
|---|---|---|
Nat Commun Dissecting the role of H3K27 acetylation and methylation in PRC2 mediated control of cellular identity. | ||
Mol Cell PALI1 promotes tumor growth through competitive recruitment of PRC2 to G9A-target chromatin for dual epigenetic silencing | ||
Mol Cell An H3K36 methylation-engaging Tudor motif of polycomb-like proteins mediates PRC2 complex targeting.
| ||
Front Immunol Comprehensive Pan-Cancer Genomic Analysis Reveals PHF19 as a Carcinogenic Indicator Related to Immune Infiltration and Prognosis of Hepatocellular Carcinoma. | ||
Brain Res Bull PDGF-BB mitigates pericyte injury by activating the PHF19-PRC2 complex via the miR-221/BRCA1 signaling axis in Alzheimer's disease. | ||
Pathology High PHD finger protein 19 expression predicts inferior prognosis in diffuse large B-cell lymphoma |
