PHF24 Polyclonal antibody

PHF24 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27959-1-AP

Synonyms

DKFZp434I2112, GINIP, KIAA1045, PHF24, Protein KIAA1045, RP11 392A14.4

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Validation Data Gallery

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  IHC staining of mouse brain using 27959-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27959-1-AP (PHF24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 27959-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27959-1-AP (PHF24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 27959-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PHF24 antibody (27959-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 27959-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PHF24 antibody (27959-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27959-1-AP targets PHF24 in IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHF24 fusion protein Ag27492
• Full Name: KIAA1045
• Calculated Molecular Weight: 45 aa
• GenBank Accession Number: NM_001304333
• Gene Symbol: KIAA1045
• Gene ID (NCBI): 23349
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.