PHGDH Polyclonal antibody

• Featured Product
• KD/KO Validated

PHGDH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

54

Cat no : 14719-1-AP

Synonyms

EC:1.1.1.399, EC:1.1.1.37, D-3-phosphoglycerate dehydrogenase, 3-PGDH, 3PGDH

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Validation Data Gallery

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  WB analysis of HEK-293 using 14719-1-AP

  WB result of PHGDH antibody (14719-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHGDH transfected HEK-293 cells.

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  WB analysis using 14719-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14719-1-AP (PHGDH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14719-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14719-1-AP (PHGDH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14719-1-AP

  IP result of anti-PHGDH (IP:14719-1-AP, 4ug; Detection:14719-1-AP 1:10000) with HeLa cells lysate 1880 ug.

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  IHC staining of human lymphoma using 14719-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14719-1-AP (PHGDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 14719-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14719-1-AP (PHGDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 14719-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14719-1-AP (PHGDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 14719-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14719-1-AP (PHGDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14719-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14719-1-AP (PHGDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14719-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14719-1-AP (PHGDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14719-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14719-1-AP (PHGDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14719-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14719-1-AP (PHGDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14719-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14719-1-AP (PHGDH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HEK-293 cells, mouse brain tissue, HeLa cells, HepG2 cells, MCF-7 cells, MDA-MB-453s cells, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lymphoma tissue, human breast hyperplasia tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 48 publications below
• IHC: See 18 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Product Information

14719-1-AP targets PHGDH in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHGDH fusion protein Ag6445
• Full Name: phosphoglycerate dehydrogenase
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC000303
• Gene Symbol: PHGDH
• Gene ID (NCBI): 26227
• RRID: AB_2283938
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ).

Publications

Species	Application	Title
human,mouse	IHC	Cell Metab; PHGDH expression increases with progression of Alzheimer's disease pathology and symptoms.; Authors - Xu Chen
mouse	WB	Nat Immunol; PERK is a critical metabolic hub for immunosuppressive function in macrophages.; Authors - Lydia N Raines
human,mouse	IHC,CoIP	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
mouse	WB,IF	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
human	WB,IHC	Mol Cancer; CircMYH9 drives colorectal cancer growth by regulating serine metabolism and redox homeostasis in a p53-dependent manner; Authors - Xin Liu
human	WB	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao