PHIP Polyclonal antibody

PHIP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 20933-1-AP

Synonyms

ndrp, PH interacting protein, PHIP

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Validation Data Gallery

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  WB analysis using 20933-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20933-1-AP (PHIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 20933-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 20933-1-AP (PHIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 20933-1-AP

  IP result of anti-PHIP (IP:20933-1-AP, 4ug; Detection:20933-1-AP 1:3000) with A375 cells lysate 3600ug.

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  IF Staining of MCF-7 using 20933-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 20933-1-AP (PHIP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, A375 cells, Jurkat cells, MCF-7 cells
• Positive IP detected in: A375 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

20933-1-AP targets PHIP in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHIP fusion protein Ag14968
• Full Name: pleckstrin homology domain interacting protein
• Calculated Molecular Weight: 1821 aa, 207 kDa
• Observed Molecular Weight: 207 kDa
• GenBank Accession Number: BC008909
• Gene Symbol: PHIP
• Gene ID (NCBI): 55023
• RRID: AB_10733522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHIP is a protein that binds to the insulin receptor substrate 1 protein and regulates glucose transporter translocation in skeletal muscle cells. PHIP may also regulate growth and survival of pancreatic beta cells. Elevated copy number of this gene may be associated with melanoma severity and the encoded protein may promote melanoma metastasis in human patients.

Publications

Species	Application	Title
human	WB,IF	Cell Metab; Exome Sequencing Identifies Genes and Gene Sets Contributing to Severe Childhood Obesity, Linking PHIP Variants to Repressed POMC Transcription.; Authors - Gaëlle Marenne
human	ChIP	Cell Commun Signal; RepID represses megakaryocytic differentiation by recruiting CRL4A-JARID1A at DAB2 promoter; Authors - Jae-Hyun Jo
human	WB	BMC Cancer; LINC00955 suppresses colorectal cancer growth by acting as a molecular scaffold of TRIM25 and Sp1 to Inhibit DNMT3B-mediated methylation of the PHIP promoter; Authors - Ganglin Ren