PHKA2 Polyclonal antibody

PHKA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24658-1-AP

Synonyms

GSD9A, PHK, PHKA2, PHKLA, PYK, PYKL, XLG, XLG2

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Validation Data Gallery

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  WB analysis using 24658-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24658-1-AP (PHKA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 24658-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24658-1-AP (PHKA2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 24658-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24658-1-AP (PHKA2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 24658-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PHKA2 antibody (24658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, SMMC-7721 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24658-1-AP targets PHKA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHKA2 fusion protein Ag9473
• Full Name: phosphorylase kinase, alpha 2 (liver)
• Calculated Molecular Weight: 138 kDa
• Observed Molecular Weight: 138 kDa
• GenBank Accession Number: BC014036
• Gene Symbol: PHKA2
• Gene ID (NCBI): 5256
• RRID: AB_2879659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		J Clin Invest; Imbalanced mitochondrial function provokes heterotaxy via aberrant ciliogenesis.; Authors - Martin D Burkhalter