PHLDB1 Polyclonal antibody

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• PHLDB1 Polyclonal antibody (33225-1-AP)

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  WB analysis using 33225-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33225-1-AP (PHLDB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 33225-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 33225-1-AP (PHLDB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 33225-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 33225-1-AP (PHLDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 33225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 33225-1-AP (PHLDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 33225-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PHLDB1 antibody (33225-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PHLDB1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 33225-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DLNB07, KIAA0638, LL5A, Pleckstrin Homology Like Domain Family B Member 1, Pleckstrin homology-like domain family B member 1

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Tested Applications

• Positive WB detected in: K-562 cells, MG-63 cells, U2OS cells
• Positive IHC detected in: human ovary tumor tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33225-1-AP targets PHLDB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag37254

  Product name: Recombinant human PHLDB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 620-800 aa of BC098586

  Sequence: LCAEYSRADGGPEAGELPSIGEATAALALAGRRPSRGLAGASGRSSEEPGVATQRLWESMERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGHVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVALETGIQKERDKEAEALET

• Full Name: pleckstrin homology-like domain, family B, member 1
• Observed Molecular Weight: 151 kDa
• GenBank Accession Number: BC098586
• Gene Symbol: PHLDB1
• Gene ID (NCBI): 23187
• RRID: AB_3742903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q86UU1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHLDB1 (Pleckstrin Homology-like Domain Family B Member 1), also known as LL5α, is a scaffolding protein that specifically binds to phosphatidylinositol-3,4,5-trisphosphate (PIP3) through its C-terminal PH domain, anchoring microtubule plus ends to the cell cortex. Functionally, PHLDB1 acts as an insulin-responsive protein that translocates from the cytoplasm to the plasma membrane upon insulin stimulation, promoting GLUT4 translocation and glucose uptake by enhancing Akt phosphorylation. In addition, PHLDB1 plays a key role in cell migration. Studies have shown that its genetic polymorphisms are associated with glioma risk, and by enhancing PI3K/AKT signaling, it promotes tumor cell invasion and metastasis, making it a potential prognostic marker and therapeutic target.