Filter: AllWBIHC

WB analysis of A431 using 27940-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 27940-1-AP (PHLDB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 27940-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 27940-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 27940-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 27940-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 27940-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 27940-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 27940-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PHLDB2 Polyclonal antibody

PHLDB2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27940-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Protein LL5-beta, Pleckstrin homology-like domain family B member 2, LL5beta, LL5B

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A431 cells
Positive IHC detected in	human skin cancer tissue, human placenta tissue, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27940-1-AP targets PHLDB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag27331 Product name: Recombinant human PHLDB2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC142678 Sequence: MEEHSYIQKELDLQNGSLEEDSVVHSVENDSQNMMESLSPKKYSSSLRFKANGDYSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGSKQFSYDGTDKNIPMKPPTPLLNTTSSLSGYPLGRADFDHYTGRDSERALRLSEKPPYSKYSSRHKSHDNVYSLGGLEGRKASGSLLAMWNGSSLSDAGPPPISRSGAASMPSSPKQARKMSIQDSLALQPKLTRHKELASENINLRTRKYSSSSLSHMGAYSRSLPRLYRATENQLTPLSLPPRNSLGNSKRTKLGEKDLPHSVIDNDNYLNFSSLSSGALPYKTSASEGNPYVSSTLSVPASPRVARKMLLASTSSCA Predict reactive species
Full Name	pleckstrin homology-like domain, family B, member 2
Calculated Molecular Weight	1253 aa, 142 kDa
Observed Molecular Weight	150 kDa
GenBank Accession Number	BC142678
Gene Symbol	PHLDB2
Gene ID (NCBI)	90102
RRID	AB_2935465
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86SQ0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The pleckstrin homology-like domain (PHLD) class of proteins are multifunctional proteins comprised of two families of proteins, PHLDA and PHLDB, each with 3 members: PHLDA1, PHLDA2, PHDLA3, PHLDB1, PHLDB2, and PHLDB3 (PMID: 32429563). All the family members possess a pleckstrin homology (PH) domain. PHLB2, also known as LL5-beta, contains two predicted coiled-coil domains, a serine-rich region predicted to contain multiple protein kinase C phosphorylation sites in addition to a PH domain (PMID: 15851520). PHLB2 is a filamin-binding protein that is an effector of the PI3K signaling pathway (PMID: 17174070). It is a PtdIns(3,4,5)P3 (PIP3) binding protein and can translocate to the plasma membrane in a receptor and PI3K-dependant manner (PMID: 12376540). PHLB2 has also been reported as a component of the postsynaptic apparatus and a regulator of postsynaptic differentiation (PMID: 15851520).