PHOX2A Polyclonal antibody

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  WB analysis of SH-SY5Y using 25804-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25804-1-AP (PHOX2A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25804-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25804-1-AP (PHOX2A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

PHOX2A Polyclonal Antibody for WB, ELISA

Cat No. 25804-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ARIX, ARIX1 homeodomain protein, CFEOM2, FEOM2, paired like homeobox 2a, PHOX2A, PMX2A

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25804-1-AP targets PHOX2A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22873

  Product name: Recombinant human PHOX2A protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 151-284 aa of BC041564

  Sequence: AASAKGAAGAAGAKKGEARCSSEDDDSKESTCSPTPDSTASLPPPPAPGLASPRLSPSPLPVALGSGPGPGPGPQPLKGALWAGVAGGGGGGPGAGAAELLKAWQPAESGPGPFSGVLSSFHRKPGPALKTNLF

• Full Name: paired-like homeobox 2a
• Calculated Molecular Weight: 284 aa, 30 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC041564
• Gene Symbol: PHOX2A
• Gene ID (NCBI): 401
• RRID: AB_2880244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14813
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.