PHYHIP Polyclonal antibody

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  WB analysis of mouse brain using 16984-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16984-1-AP (PHYHIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PHYHIP Polyclonal Antibody for WB, ELISA

Cat No. 16984-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

DYRK1AP3, KIAA0273, PAHX AP, PAHX AP1, PAHXAP1, PHYHIP

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Tested Applications

• Positive WB detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16984-1-AP targets PHYHIP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10575

  Product name: Recombinant human PHYHIP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-330 aa of BC034034

  Sequence: MELLSTPHSIEINNITCDSFSISWAMEDSDLERVTHYFIDLNKKENKNSNKFKHRDVPTKLVAKAVPLPMTVRGHWFLSPRTEYSVAVQTAVKQSDGEYLVSGWSETVEFCTGDYAKEHLAQLQEKAEQIAGRMLRFSVFYRNHHKEYFEHARTHCGNMLQPYLKDNSGSHGSPTSGMLHGVFFSCNTEFNTGQPPQDSPYGRWRFQIPAQRLFNPSTNLYFADFYCMYTAYHYAILVLAPKGSMGDRFCRDRRPLLDIACNKFLTCSVEDGELVFRHAQDLILEIIYTEPVDLSLGTLGEISGHQLMSLSTADAKKDPSCKTCNISVGR

• Full Name: phytanoyl-CoA 2-hydroxylase interacting protein
• Calculated Molecular Weight: 330 aa, 38 kDa
• Observed Molecular Weight: 35 kDa, 43-45 kDa
• GenBank Accession Number: BC034034
• Gene Symbol: PHYHIP
• Gene ID (NCBI): 9796
• RRID: AB_2163759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92561
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHYHIP, also named as DYRK1AP3 and KIAA0273, belongs to the PHYHIP family. Its interaction with PHYH suggests a role in the development of the central system. This antibody recognizes both PHYHIP (35-38 kDa) and PHYHIPL (43-45 kDa).