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PHYHIPL Polyclonal antibody, PBS Only (16937-1-PBS)

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WB analysis using 16937-1-AP (same clone as 16937-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16937-1-AP (PHYHIPL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16937-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 16937-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 16937-1-AP (same clone as 16937-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16937-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 16937-1-AP (same clone as 16937-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16937-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 16937-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 16937-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 16937-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 16937-1-AP (same clone as 16937-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 16937-1-AP (PHYHIPL antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16937-1-PBS in a different storage buffer formulation.

PHYHIPL Polyclonal antibody, PBS Only

Name: PHYHIPL Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 16937-1-PBS

PHYHIPL Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 16937-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

KIAA1796, Phytanoyl-CoA hydroxylase-interacting protein-like, UNQ6309/PRO20934

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

16937-1-PBS targets PHYHIPL in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10435 Product name: Recombinant human PHYHIPL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-376 aa of BC011268 Sequence: MEVPRLDHALNSPTSPCEEVIKNLSLEAIQLCDRDGNKSQDSGIAEMEELPVPHNIKISNITCDSFKISWEMDSKSKDRITHYFIDLNKKENKNSNKFKHKDVPTKLVAKAVPLPMTVRGHWFLSPRTEYTVAVQTASKQVDGDYVVSEWSEIIEFCTADYSKVHLTQLLEKAEVIAGRMLKFSVFYRNQHKEYFDYVREHHGNAMQPSVKDNSGSHGSPISGKLEGIFFSCSTEFNTGKPPQDSPYGRYRFEIAAEKLFNPNTNLYFGDFYCMYTAYHYVILVIAPVGSPGDEFCKQRLPQLNSKDNKFLTCTEEDGVLVYHHAQDVILEVIYTDPVDLSLGTVAEITGHQLMSLSTANAKKDPSCKTCNISVGR Predict reactive species
Full Name	phytanoyl-CoA 2-hydroxylase interacting protein-like
Calculated Molecular Weight	376 aa, 43 kDa
Observed Molecular Weight	39 kDa and 42-45 kDa
GenBank Accession Number	BC011268
Gene Symbol	PHYHIPL
Gene ID (NCBI)	84457
RRID	AB_2163761
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96FC7
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PHYHIPL, a kind of mitochondrial protein, is associated with energy production, oxidative stress and organelle dynamics. It is reported that global ischemia is related to the change of PHYHIPL protein level. Compared with the control group, the level of APOE (may be involved in abnormal regulation of mitochondrial function) is significantly increased after ischemic stimulation, while the APOE genotype has a effect on PHYHIPL protein level. 16937-1-AP detects 39 kDa isoform and 42-45 kDa isoform in SDS-PAGE. (PMID: 21878944)