Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20662-1-AP targets PI3 Kinase p110 Gamma in WB, IHC, IP, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | phosphoinositide-3-kinase, catalytic, gamma polypeptide |
| Calculated Molecular Weight | 126 kDa |
| Observed Molecular Weight | 100-130 kDa |
| GenBank Accession Number | NM_002649 |
| Gene Symbol | PIK3CG |
| Gene ID (NCBI) | 5294 |
| RRID | AB_2878720 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48736 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PI3 Kinase p110 Gamma antibody 20662-1-AP | Download protocol |
| IP protocol for PI3 Kinase p110 Gamma antibody 20662-1-AP | Download protocol |
| WB protocol for PI3 Kinase p110 Gamma antibody 20662-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PI3 Kinase p110 Gamma Polyclonal antibody (Cat# 20662-1-AP) has 8 citations published in Nature Communications, Phytomedicine, International Journal of Biological Macromolecules, EMBO Molecular Medicine, Biofactors, American Journal of Pathology, and American Journal of Physiology Endocrinology and Metabolism. Research fields span cardiotoxicity and immunotherapy, traditional Chinese medicine pharmacology, ischemic stroke, gut-brain axis neuroscience, cardiac hypertrophy, bladder cancer, heterotopic ossification, and epigenetic regulation of glucose metabolism.
| Species | Application | Title |
|---|---|---|
Nat Commun Microbiota-indole-3-propionic acid-heart axis mediates the protection of leflunomide against αPD1-induced cardiotoxicity in mice | ||
Phytomedicine Integrating serum pharmacochemistry, network pharmacology, and metabolomics to explore the protective mechanism of Hua-Feng-Dan in ischemic stroke. | ||
Phytomedicine Chinese medicine Sheng Jing Decoction alleviates oligoasthenospermia by modulating PI3K/Akt and MAPK signaling pathways. | ||
Int J Biol Macromol Water-insoluble dietary fiber from walnut relieves constipation through Limosilactobacillus reuteri-mediated serotonergic synapse and neuroactive ligand-receptor pathways | ||
EMBO Mol Med RBMS1 orchestrates cardiac hypertrophy by facilitating CTTN splice-switching and sarcomere dynamics. | ||
Biofactors Knockdown of integrin β1 inhibits proliferation and promotes apoptosis in bladder cancer cells |
