PI3 Kinase p110 Gamma Polyclonal antibody

PI3 Kinase p110 Gamma Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Rat, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20662-1-AP

Synonyms

p120 PI3K, PI3 Kinase p110 Gamma, PI3 kinase subunit gamma, PI3CG, PI3K, PI3K gamma, PI3K p110(gamma), PI3Kgamma, PIK3, PIK3CG, PtdIns 3 kinase subunit gamma



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

20662-1-AP targets PI3 Kinase p110 Gamma in WB, IP, IHC, ELISA applications and shows reactivity with Rat, mouse samples.

Tested Reactivity Rat, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name phosphoinositide-3-kinase, catalytic, gamma polypeptide
Calculated Molecular Weight 126 kDa
Observed Molecular Weight 100-130 kDa
GenBank Accession NumberNM_002649
Gene Symbol PIK3CG
Gene ID (NCBI) 5294
RRIDAB_2878720
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PI3 Kinase p110 Gamma antibody 20662-1-APDownload protocol
IHC protocol for PI3 Kinase p110 Gamma antibody 20662-1-APDownload protocol
IP protocol for PI3 Kinase p110 Gamma antibody 20662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Am J Physiol Endocrinol Metab

Frequent and mild scrotal heat stress in mice epigenetically alters glucose metabolism in the male offspring.

Authors - Xiaoyan Wan
mouseWB

Am J Pathol

Suppression of Overactive Insulin-Like Growth Factor 1 Attenuates Trauma-Induced Heterotopic Ossification in Mice

Authors - Dong Mao