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  • KD/KO Validated

PI4KA Polyclonal antibody

PI4KA Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12411-1-AP

Synonyms

PI4 kinase alpha, PI4K ALPHA, pi4K230, PI4KA, PIK4, PIK4CA, PtdIns 4 kinase alpha



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, human brain tissue, MCF-7 cells, NIH-3T3 cells (RNAi), rat brain tissue
Positive IP detected inmouse brain tissue
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12411-1-AP targets PI4KA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PI4KA fusion protein Ag3062
Full Name phosphatidylinositol 4-kinase, catalytic, alpha
Calculated molecular weight 2044 aa, 231 kDa
Observed molecular weight 231 kDa
GenBank accession numberBC018120
Gene symbol PI4KA
Gene ID (NCBI) 5297
RRIDAB_2268237
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).

Protocols

Product Specific Protocols
WB protocol for PI4KA antibody 12411-1-APDownload protocol
IHC protocol for PI4KA antibody 12411-1-APDownload protocol
IF protocol for PI4KA antibody 12411-1-APDownload protocol
IP protocol for PI4KA antibody 12411-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Authors - Jay Xiaojun Tan
  • KD Validated
humanWB

Science

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Authors - Shun Nagashima
  • KD Validated
humanWB,IF

Brain

Biallelic PI4KA variants cause a novel neurodevelopmental syndrome with hypomyelinating leukodystrophy.

Authors - Edgard Verdura
humanIF

EMBO J

Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.

Authors - Zhengming Wu
human,mouseWB,IHC,IF

Cell Rep

A Forward Genetic Screen Targeting the Endothelium Reveals a Regulatory Role for the Lipid Kinase Pi4ka in Myelo- and Erythropoiesis.

Authors - Safiyyah Ziyad
  • KD Validated
humanWB

Biomaterials

Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells.

Authors - Ming Gao
  • KD Validated