PI4KB Polyclonal antibody

• Featured Product
• KD/KO Validated

PI4KB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 13247-1-AP

Synonyms

PtdIns 4-kinase beta, PIK4CB, PI4KIII, PI4K-beta, Phosphatidylinositol 4-kinase beta

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Validation Data Gallery

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  WB analysis of K-562 using 13247-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13247-1-AP (PI4KB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH-3T3 cells (RNAi) using 13247-1-AP

  WB result of anti- PI4Kb (13247-1-AP) with NIH-3T3 cells (RNAi).

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  WB analysis of Jurkat using 13247-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13247-1-AP (PI4KB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13247-1-AP

  IP result of anti-PI4KB (IP:13247-1-AP, 4ug; Detection:13247-1-AP 1:300) with K-562 cells lysate 3600ug.

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  IHC staining of human liver using 13247-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13247-1-AP (PI4KB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 13247-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13247-1-AP (PI4KB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of C6 using 13247-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using PI4KB antibody (13247-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, NIH-3T3 cells (RNAi)
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 2 publications below

Product Information

13247-1-AP targets PI4KB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PI4KB fusion protein Ag3989
• Full Name: phosphatidylinositol 4-kinase, catalytic, beta
• Calculated Molecular Weight: 801 aa, 90 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC040300
• Gene Symbol: PI4KB
• Gene ID (NCBI): 5298
• RRID: AB_2252370
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PI4KB(phosphatidylinositol 4-kinase beta) is also named as PIK4CB, NPIK, PI4K92 and belongs to the PI3/PI4-kinase family. It may regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation and involved in Golgi-to-plasma membrane trafficking. PI4KB is a type III enzyme that is sensitive to wortmannin(PMID:9020160). This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human,mouse	WB	Cell Res; Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity; Authors - Ying Gao
human	IF	EMBO J; Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.; Authors - Zhengming Wu
human	WB	PLoS Pathog; Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.; Authors - Tao Wang
human		Virol Sin; Construction of coxsackievirus B5 viruses with luciferase reporters and their applications in vitro and in vivo; Authors - Shangrui Guo
human	WB,IF	J Virol; N-terminal acetyltransferase 6 facilitates enterovirus 71 replication by regulating PI4KB expression and replication organelle biogenesis; Authors - Hang Yang
mouse	WB	J Neurosci; PI4KIIIβ-Mediated Phosphoinositides Metabolism Regulates Function of the VTA Dopaminergic Neurons and Depression-Like Behavior; Authors - Yuqi Sang