Tested Applications
| Positive WB detected in | K-562 cells, NIH-3T3 cells (RNAi), NIH/3T3 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | C6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13247-1-AP targets PI4KB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3989 Product name: Recombinant human PI4KB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 449-801 aa of BC040300 Sequence: IGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQLAHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSPRNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGIM Predict reactive species |
| Full Name | phosphatidylinositol 4-kinase, catalytic, beta |
| Calculated Molecular Weight | 801 aa, 90 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC040300 |
| Gene Symbol | PI4KB |
| Gene ID (NCBI) | 5298 |
| RRID | AB_2252370 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBF8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PI4KB(phosphatidylinositol 4-kinase beta) is also named as PIK4CB, NPIK, PI4K92 and belongs to the PI3/PI4-kinase family. It may regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation and involved in Golgi-to-plasma membrane trafficking. PI4KB is a type III enzyme that is sensitive to wortmannin(PMID:9020160). This protein has 3 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PI4KB antibody 13247-1-AP | Download protocol |
| IHC protocol for PI4KB antibody 13247-1-AP | Download protocol |
| IP protocol for PI4KB antibody 13247-1-AP | Download protocol |
| WB protocol for PI4KB antibody 13247-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PI4KB Polyclonal antibody (Cat# 13247-1-AP) has 19 citations. They appear in journals including Cell Research, Nature Communications, Advanced Science, Science Advances, EMBO Journal, Cell Reports, Journal of Cell Biology, Frontiers in Microbiology, PLoS Pathogens, Journal of Neuroscience, Journal of Virology, Journal of Cell Science, and Cerebral Cortex. Research fields span lipid/phosphoinositide metabolism, autophagy, ciliogenesis, endosomal trafficking, viral replication (EV71, coxsackievirus), cancer (esophageal, GIST, breast), neurobiology, and inflammation.
| Species | Application | Title |
|---|---|---|
Cell Res Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity | ||
Nat Commun VPS13B recruits lipid vesicles to promote mitochondrial fission and quality control. | ||
Adv Sci (Weinh) ARL5B Drives Esophageal Squamous Cell Carcinoma Progression via ROCK1-SREBP1-Mediated Lipid Metabolic Reprogramming | ||
Sci Adv Golgi-localized phosphatidylinositol 4-kinase β mediates Rab11a activation and trafficking to promote ciliogenesis.
| ||
Phytomedicine Curculigoside A alleviates metabolic dysfunction-associated steatohepatitis by targeting Rab30 to improve lipid homeostasis. | ||
EMBO J Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB. |
