PIAS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PIAS1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, coIP, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 23395-1-AP

Synonyms

DDXBP1, DEAD/H box binding protein 1, E3 SUMO protein ligase PIAS1, Gu binding protein, GU/RH II, PIAS1, ZMIZ3

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Validation Data Gallery

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  WB analysis using 23395-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23395-1-AP (PIAS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 23395-1-AP

  IP result of anti-PIAS1 (IP:23395-1-AP, 4ug; Detection:23395-1-AP 1:500) with HepG2 cells lysate 2520 ug.

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  IHC staining of mouse testis using 23395-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23395-1-AP (PIAS1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 23395-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23395-1-AP (PIAS1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, HeLa cells, Jurkat cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 23395-1-AP is PIAS1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIAS1 fusion protein Ag18132
• Full Name: protein inhibitor of activated STAT, 1
• Calculated Molecular Weight: 651 aa, 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC121797
• Gene Symbol: PIAS1
• Gene ID (NCBI): 8554
• RRID: AB_2879272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; CryoET reveals organelle phenotypes in huntington disease patient iPSC-derived and mouse primary neurons; Authors - Gong-Her Wu
human	WB	Nat Commun; Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin; Authors - Tao Zhang
mouse,rat	WB,coIP	J Hazard Mater; Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.; Authors - Junrong Wu
human	WB,IF	PLoS Pathog; PIAS1-mediated SUMOylation of influenza A virus PB2 restricts viral replication and virulence.; Authors - Guangwen Wang ; KD Validated
human	WB	Mol Med Rep; Role of SUMOylation of STAT1 in tubular epithelial‑mesenchymal transition induced by high glucose; Authors - Cunyang Gu
human	IHC, WB	DNA Cell Biol; miR-182-5p and miR-96-5p Target PIAS1 and Mediate the Negative Feedback Regulatory Loop between PIAS1 and STAT3 in Endometrial Cancer.; Authors - Yuzhen Xiao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Praveen (Verified Customer) (02-25-2019)

WORK WELL

• Applications: Immunoprecipitation,
• Cell Tissue Type: Ac16

PIAS1 Antibody Immunoprecipitation, validation ( dilution) in Ac16 (Cat no:23395-1-AP)