PIAS2 Polyclonal antibody

PIAS2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16074-1-AP

Synonyms

E3 SUMO-protein transferase PIAS2, E3 SUMO-protein ligase PIAS2, DIP, DAB2-interacting protein, ARIP3

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Validation Data Gallery

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  WB analysis using 16074-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16074-1-AP (PIAS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16074-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16074-1-AP (PIAS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16074-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16074-1-AP (PIAS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 16074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 16074-1-AP (PIAS2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, K-562 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 3 publications below

Product Information

16074-1-AP targets PIAS2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIAS2 fusion protein Ag9032
• Full Name: protein inhibitor of activated STAT, 2
• Calculated Molecular Weight: 63 kDa, 69 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC015190
• Gene Symbol: PIAS2
• Gene ID (NCBI): 9063
• RRID: AB_2164208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIAS2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. PIAS2 also plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. PIAS2 has 3 isoforms produced by alternative splicing with the MW of 68 kDa, 63 kDa and 45 kDa.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	WB	Biochim Biophys Acta Mol Cell Res; IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5; Authors - Junfeng Du
human	WB,IF	J Mol Med (Berl); Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.; Authors - Yuhong Zhang
human	IF	Sci Rep; Chromosome 8q24 amplification associated with human hepatocellular carcinoma predicts MYC/ZEB1/MIZ1 transcriptional regulation; Authors - Joeffrey J Chahine