PIAS4 Polyclonal antibody

PIAS4 Polyclonal Antibody for WB,IF,FC,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 18469-1-AP


E3 SUMO protein ligase PIAS4, PIAS gamma, PIAS4, Piasg, PIASY, ZMIZ6

Tested Applications

Positive WB detected inHL-60 cells, Jurkat cells, K-562 cells
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18469-1-AP targets PIAS4 in WB,IF,FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIAS4 fusion protein Ag13316
Full Name protein inhibitor of activated STAT, 4
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57-70 kDa
GenBank Accession NumberBC010047
Gene Symbol PIAS4
Gene ID (NCBI) 51588
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members(PMID:22829926). This protein can be sumoylated and Lys-35 is the main site of sumoylation(PMID:15831457).


Product Specific Protocols
WB protocol for PIAS4 antibody 18469-1-APDownload protocol
IF protocol for PIAS4 antibody 18469-1-APDownload protocol
FC protocol for PIAS4 antibody 18469-1-APDownload protocol
Standard Protocols
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