PIAS4 Recombinant monoclonal antibody

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• PIAS4 Recombinant monoclonal antibody (86793-1-RR)

PIAS4 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86793-1-RR

Clone No.251712C4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

E3 SUMO-protein ligase PIAS4, EC:2.3.2.27, PIAS gamma, PIASG, PIAS-gamma

• 

  WB analysis using 86793-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86793-1-RR (PIAS4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 86793-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86793-1-RR against Human PIAS4 were performed. The affinity constant is 0.983 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: K-562 cells, HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86793-1-RR targets PIAS4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag37383

  Product name: Recombinant human PIAS4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 210-510 aa of BC029874

  Sequence: PNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIRAEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMENGKPGADVVDLTLDSSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVPAC

• Full Name: protein inhibitor of activated STAT, 4
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC029874
• Gene Symbol: PIAS4
• Gene ID (NCBI): 51588
• RRID: AB_3745122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N2W9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members (PMID:22829926). This protein can be sumoylated and Lys-35 is the main site of sumoylation(PMID:15831457).