PICALM Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• PICALM Polyclonal antibody, PBS Only (28554-1-PBS)

• 

  WB analysis using 28554-1-AP (same clone as 28554-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28554-1-AP (PICALM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28554-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 28554-1-AP (same clone as 28554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28554-1-AP (PICALM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28554-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 28554-1-AP (same clone as 28554-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PICALM antibody (28554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28554-1-PBS in a different storage buffer formulation.

PICALM Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 28554-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CALM, CLTH, LAP, Phosphatidylinositol-binding clathrin assembly protein

---

Product Information

28554-1-PBS targets PICALM in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29505

  Product name: Recombinant human PICALM protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-356 aa of BC064357

  Sequence: MSGQSLTDRITAAQHSVTGSAVSKTVCKATTHEIMGPKKKHLDYLIQCTNEMNVNIPQLADSLFERTTNSSWVVVFKSLITTHHLMVYGNERFIQYLASRNTLFNLSNFLDKSGLQGYDMSTFIRRYSRYLNEKAVSYRQVAFDFTKVKRGADGVMRTMNTEKLLKTVPIIQNQMDALLDFNVNSNELTNGVINAAFMLLFKDAIRLFAAYNEGIINLLEKYFDMKKNQCKEGLDIYKKFLTRMTRISEFLKVAEQVGIDRGDIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRLKELAKKPHTSLTT

• Full Name: phosphatidylinositol binding clathrin assembly protein
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC064357
• Gene Symbol: PICALM
• Gene ID (NCBI): 8301
• RRID: AB_2918175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13492
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS). (PMID: 33397371, PMID: 26075887, PMID: 26005850)