PID1 Polyclonal antibody

• 

  WB analysis using 33859-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33859-1-AP (PID1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

PID1 Polyclonal Antibody for WB, ELISA

Cat No. 33859-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

---

Tested Applications

• Positive WB detected in: rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33859-1-AP targets PID1 in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag40871

  Product name: Recombinant human PID1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-168 aa of BC040164

  Sequence: MWQPATERLQVTYLGKVSTTGMQFLSGCTEKPVIELWKKHTLAREDVFPANALLEIRPFQVWLHHLDHKGEATVHMDTFQVARIAYCTADHNVSPNIFAWVYREINDDLSYQMDCHAVECESKLEAKKLAHAMMEAFRKTFHSMKSDGRIHSNSSSEEVSQELESDDG

• Full Name: phosphotyrosine interaction domain containing 1
• Calculated Molecular Weight: 168 aa, 19 kDa
• Observed Molecular Weight: 25 kDa, 28 kDa
• GenBank Accession Number: BC040164
• Gene Symbol: PID1
• Gene ID (NCBI): 55022
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q7Z2X4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PID1 is a cytoplasmic PTB-domain adaptor that negatively regulates insulin and IGF1 signaling, modulating AKT activity, glucose uptake, and mitochondrial function. Enriched in muscle, adipose tissue, and liver, PID1 promotes insulin resistance, impacts lipid handling, and contributes to metabolic disease. Its dysregulation also influences tumor metabolism and stress responses, underscoring PID1 as an important node linking growth factor signaling, energy homeostasis, and pathophysiology.