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PIGA Polyclonal antibody, PBS Only (13679-1-PBS)

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WB analysis of HeLa using 13679-1-AP (same clone as 13679-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 13679-1-AP (PIGA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13679-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 13679-1-AP (same clone as 13679-1-PBS)
WB result of PIGA antibody (13679-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIGA transfected HEK-293 cells. This data was developed using the same antibody clone with 13679-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 13679-1-AP (same clone as 13679-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 13679-1-AP (PIGA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13679-1-PBS in a different storage buffer formulation.
IHC staining of mouse lung using 13679-1-AP (same clone as 13679-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13679-1-PBS in a different storage buffer formulation.
IHC staining of mouse lung using 13679-1-AP (same clone as 13679-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13679-1-PBS in a different storage buffer formulation.
IHC staining of rat liver using 13679-1-AP (same clone as 13679-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13679-1-PBS in a different storage buffer formulation.
IHC staining of rat liver using 13679-1-AP (same clone as 13679-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13679-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PIGA Polyclonal antibody, PBS Only

Name: PIGA Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 13679-1-PBS

PIGA Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 13679-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

13679-1-PBS targets PIGA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4530 Product name: Recombinant human PIGA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-421 aa of BC038236 Sequence: MACRGGAGNGHRASATLSRVSPGSLYTCRTRTHNICMVSDFFYPNMGGVESHIYQLSQCLIERGHKVIIVTHAYGNRKGIRYLTSGLKVYYLPLKVMYNQSTATTLFHSLPLLRYIFVRERVTIIHSHSSFSAMAHDALFHAKTMGLQTVFTDHSLFGFADVSSVLTNKLLTVSLCDTNHIICVSYTSKENTVLRAALNPEIVSVIPNAVDPTDFTPDPFRRHDSITIVVVSRLVYRKGIDLLSGIIPELCQKYPDLNFIIGGEGPKRIILEEVRERYQLHDRVRLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTRVGGIPEVLPENLIILCEPSVKSLCEGLEKAIFQLKSGTLPAPENIHNIVKTFYTWRNVAERTEKVYDRVSVEAVLPMDKRLDRLISHCG Predict reactive species
Full Name	phosphatidylinositol glycan anchor biosynthesis, class A
Calculated Molecular Weight	484 aa, 54 kDa
Observed Molecular Weight	38 kDa
GenBank Accession Number	BC038236
Gene Symbol	PIGA
Gene ID (NCBI)	5277
RRID	AB_2164656
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P37287
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.