PIGT Polyclonal antibody

• Featured Product
• KD/KO Validated

PIGT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16906-1-AP

Synonyms

UNQ716/PRO1379, PSEC0163, Phosphatidylinositol-glycan biosynthesis class T protein, NDAP, GPI transamidase component PIG-T

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Validation Data Gallery

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  WB analysis of human brain using 16906-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16906-1-AP (PIGT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 16906-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16906-1-AP (PIGT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 16906-1-AP

  IP result of anti-PIGT (IP:16906-1-AP, 4ug; Detection:16906-1-AP 1:300) with mouse liver tissue lysate 6000 ug.

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  IHC staining of human brain using 16906-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16906-1-AP (PIGT antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 16906-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16906-1-AP (PIGT antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 16906-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16906-1-AP (PIGT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16906-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16906-1-AP (PIGT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue, A549 cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

16906-1-AP targets PIGT in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIGT fusion protein Ag10521
• Full Name: phosphatidylinositol glycan anchor biosynthesis, class T
• Calculated Molecular Weight: 578 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC015022
• Gene Symbol: PIGT
• Gene ID (NCBI): 51604
• RRID: AB_2164836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIGT is a subunit of the glycosylphosphatidylinositol transamidase complex that catalyzes the attachment of proteins to GPI-anchors. GPI-anchors are glycolipids found on membrane of diverse cells that help proteins anchoring to the cell surface. Mutations of PIGT have been linked to developmental disorders including multiple congenital anomalies-hypotonia-seizures syndrome-3. Multiple isoforms of PIGT exist due to the alternative splicing, with the predicted MWs ranging from 42 kDa to 66 kDa.

Publications

Species	Application	Title
human	WB	J Transl Med; PIGT promotes cell growth, glycolysis, and metastasis in bladder cancer by modulating GLUT1 glycosylation and membrane trafficking; Authors - Mingyue Tan ; KD Validated
mouse	WB,IF	CNS Neurosci Ther; Integrated transcriptomic and proteomic profiling reveals the key molecular signatures of brain endothelial reperfusion injury; Authors - Yabin Ji
human,mouse	WB	Nat Commun; Proteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its role in glycosylphosphatidylinositol-anchored protein biogenesis; Authors - Xiaoqiong Wei