Tested Applications
| Positive WB detected in | HeLa cells, PC-3 cells, human placenta tissue |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | mouse ovary tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22028-1-AP targets PIK3C2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16894 Product name: Recombinant human PIK3C2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-338 aa of BC113658 Sequence: MAQISSNSGFKECPSSHPEPTRAKDVDKEEALQMEAEALAKLQKDRQVTDNQRGFELSSSTRKKAQVYNKQDYDLMVFPESDSQKRALDIDVEKLTQAELEKLLLDDSFETKKTPVLPVTPILSPSFSAQLYFRPTIQRGQWPPGLPGPSTYALPSIYPSTYSKQAAFQNGFNPRMPTFPSTEPIYLSLPGQSPYFSYPLTPATPFHPQGSLPIYRPVVSTDMAKLFDKIASTSEFLKNGKARTDLEITDSKVSNLQVSPKSEDISKFDWLDLDPLSKPKVDNVEVLDHEEEKNVSSLLAKDPWDAVLLEERSTANCHLERKVNGKSLSVATVTRSQS Predict reactive species |
| Full Name | phosphoinositide-3-kinase, class 2, alpha polypeptide |
| Calculated Molecular Weight | 1686 aa, 191 kDa |
| Observed Molecular Weight | 191 kDa |
| GenBank Accession Number | BC113658 |
| Gene Symbol | PIK3C2A |
| Gene ID (NCBI) | 5286 |
| RRID | AB_11183760 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00443 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PIK3C2A antibody 22028-1-AP | Download protocol |
| IHC protocol for PIK3C2A antibody 22028-1-AP | Download protocol |
| IP protocol for PIK3C2A antibody 22028-1-AP | Download protocol |
| WB protocol for PIK3C2A antibody 22028-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PIK3C2A Polyclonal antibody (catalog no. 22028-1-AP) has 15 citations. These appear in journals including Cancer Cell, Science, Cell Research, Nature Cell Biology, Advanced Science, Cell Communication and Signaling, Journal of Experimental Medicine, Cell Death Discovery, Frontiers in Cellular and Infection Microbiology, Journal of Cell Science, Scientific Reports, Journal of Cardiovascular Development and Diseases, and Oncotarget. Research fields span breast and lung cancer, mitotic spindle dynamics, phosphoinositide signaling, autophagy, metastasis, and cardiovascular disease.
| Species | Application | Title |
|---|---|---|
Cancer Cell Mitotic Spindle Assembly and Genomic Stability in Breast Cancer Require PI3K-C2α Scaffolding Function. | ||
Science PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation.
| ||
Cell Res Phosphatidylinositol 3,4-bisphosphate regulates neurite initiation and dendrite morphogenesis via actin aggregation. | ||
Nat Cell Biol Kinase-independent synthesis of 3-phosphorylated phosphoinositides by a phosphotransferase
| ||
Adv Sci (Weinh) Phosphoinositide Conversion Inactivates R-RAS and Drives Metastases in Breast Cancer. | ||
J Exp Med PTPN23-dependent activation of PI3KC2α is a therapeutic vulnerability of BRAF-mutant cancers. |
Reviews
What customers say
The single review for this antibody (rated 5/5) reports excellent performance in both Western Blot and Immunofluorescence using cancer cell lines at a 1:1000 dilution. The reviewer highlights brilliant WB band quality and high IF specificity. The only suggestion was a wish for additional pricing discounts. Overall, the feedback is strongly positive regarding product quality and specificity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
HUAYI (Verified Customer) (01-17-2020) | This antibody provide brilliant Western Blot results, and as well, the specificity for Immunofloruorence is high. But we are glad if more discounts would be received.
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