PIK3C2A Polyclonal antibody

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  WB analysis of HeLa using 22028-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22028-1-AP

  WB result of PIK3C2A antibody (22028-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIK3C2A transfected HeLa cells.

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  WB analysis of PC-3 using 22028-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22028-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 22028-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 22028-1-AP

  IP result of anti-PIK3C2A (IP:22028-1-AP, 4ug; Detection:22028-1-AP 1:15000) with PC-3 cells lysate 1480 ug.

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  IHC staining of mouse ovary using 22028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 22028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 22028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 22028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 22028-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PIK3C2A antibody (22028-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PIK3C2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22028-1-AP

Publications(11)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:2.7.1.137, Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, Phosphoinositide 3-kinase-C2-alpha, PI3 K C2A, PI3K C2 alpha

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells, human placenta tissue
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: mouse ovary tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Product Information

22028-1-AP targets PIK3C2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16894

  Product name: Recombinant human PIK3C2A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-338 aa of BC113658

  Sequence: MAQISSNSGFKECPSSHPEPTRAKDVDKEEALQMEAEALAKLQKDRQVTDNQRGFELSSSTRKKAQVYNKQDYDLMVFPESDSQKRALDIDVEKLTQAELEKLLLDDSFETKKTPVLPVTPILSPSFSAQLYFRPTIQRGQWPPGLPGPSTYALPSIYPSTYSKQAAFQNGFNPRMPTFPSTEPIYLSLPGQSPYFSYPLTPATPFHPQGSLPIYRPVVSTDMAKLFDKIASTSEFLKNGKARTDLEITDSKVSNLQVSPKSEDISKFDWLDLDPLSKPKVDNVEVLDHEEEKNVSSLLAKDPWDAVLLEERSTANCHLERKVNGKSLSVATVTRSQS

• Full Name: phosphoinositide-3-kinase, class 2, alpha polypeptide
• Calculated Molecular Weight: 1686 aa, 191 kDa
• Observed Molecular Weight: 191 kDa
• GenBank Accession Number: BC113658
• Gene Symbol: PIK3C2A
• Gene ID (NCBI): 5286
• RRID: AB_11183760
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00443
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Science; PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation.; Authors - Federico Gulluni ; KD Validated
human	WB,IF	Cancer Cell; Mitotic Spindle Assembly and Genomic Stability in Breast Cancer Require PI3K-C2α Scaffolding Function.; Authors - Federico Gulluni
rat	WB,IF	Cell Res; Phosphatidylinositol 3,4-bisphosphate regulates neurite initiation and dendrite morphogenesis via actin aggregation.; Authors - Shu-Xin Zhang
human	WB,IF	J Cell Sci; Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization; Authors - Ellis L Ryan ; KD Validated
human	WB,IF	J Cell Sci; Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization.; Authors - Ellis L Ryan
human	WB	Front Cell Infect Microbiol; The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection.; Authors - Teng Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

HUAYI (Verified Customer) (01-17-2020)

This antibody provide brilliant Western Blot results, and as well, the specificity for Immunofloruorence is high. But we are glad if more discounts would be received.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cancer cell lines

PIK3C2A Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in cancer cell lines (Cat no:22028-1-AP)