PIK3C2A Recombinant monoclonal antibody, PBS Only (Detector)

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• PIK3C2A Recombinant monoclonal antibody, PBS Only (Detector) (88380-3-PBS)

PIK3C2A Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 88380-3-PBS

Clone No.260864A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

• 

  Sandwich ELISA sample test of MP03538-1

  The mean PIK3C2A concentration was determined to be 11.0 ng/mL in HaCaT cell extract based on a 1.2 mg/mL extract load, 9.6 ng/mL in PC-3 cell extract based on a 1.2 mg/mL extract load and 3.4 ng/mL in HT-1080 cell extract based on a 1.2 mg/mL extract load.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA sample test of MP03538-1

  The mean PIK3C2A concentration was determined to be 3.1 ng/mL in THP-1 cell extract based on a 1.2 mg/mL extract load, 4.0 ng/mL in MG-63 cell extract based on a 1.2 mg/mL extract load and 3.4 ng/mL in HL-60 cell extract based on a 1.2 mg/mL extract load.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA sample test of MP03538-1

  The mean PIK3C2A concentration was determined to be 16.3 ng/mL in HT-29 cell extract based on a 1.2 mg/mL extract load, 7.3 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load and 10.5 ng/mL in RT-4 cell extract based on a 1.2 mg/mL extract load.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA standard curve of MP03538-1

  Sandwich ELISA standard curve of MP03538-1, Human PIK3C2A Recombinant Matched Antibody Pair - PBS only. 88380-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16894. 88380-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Cytometric bead array sample test of MP03538-1

  The mean PIK3C2A concentration was determined to be 10.2 ng/mL in Caco-2 cell extract based on a 1.2 mg/mL extract load, 15.5 ng/mL in HT-29 cell extract based on a 1.2 mg/mL extract load, 3.0 ng/mL in HL-60 cell extract based on a 1.2 mg/mL extract load.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Cytometric bead array standard curve of MP03538-1

  Cytometric bead array standard curve of MP03538-1, PIK3C2A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88380-1-PBS. Detection antibody: 88380-3-PBS. Standard: Ag16894. Range: 0.156-20 ng/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

88380-3-PBS targets PIK3C2A as part of a matched antibody pair:

MP03538-1: 88380-1-PBS capture and 88380-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag16894

  Product name: Recombinant human PIK3C2A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-338 aa of BC113658

  Sequence: MAQISSNSGFKECPSSHPEPTRAKDVDKEEALQMEAEALAKLQKDRQVTDNQRGFELSSSTRKKAQVYNKQDYDLMVFPESDSQKRALDIDVEKLTQAELEKLLLDDSFETKKTPVLPVTPILSPSFSAQLYFRPTIQRGQWPPGLPGPSTYALPSIYPSTYSKQAAFQNGFNPRMPTFPSTEPIYLSLPGQSPYFSYPLTPATPFHPQGSLPIYRPVVSTDMAKLFDKIASTSEFLKNGKARTDLEITDSKVSNLQVSPKSEDISKFDWLDLDPLSKPKVDNVEVLDHEEEKNVSSLLAKDPWDAVLLEERSTANCHLERKVNGKSLSVATVTRSQS

• Full Name: phosphoinositide-3-kinase, class 2, alpha polypeptide
• Calculated Molecular Weight: 1686 aa, 191 kDa
• GenBank Accession Number: BC113658
• Gene Symbol: PIK3C2A
• Gene ID (NCBI): 5286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00443
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.