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VPS34 Polyclonal antibody

VPS34 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13723-1-AP

Synonyms

hVps34, PI 3 Kinase Class 3, PI3K p100, PI3K type 3, PIK3C3, PtdIns 3 kinase type 3, VPS34



Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue, mouse lung tissue, Jurkat cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

13723-1-AP targets VPS34 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name phosphoinositide-3-kinase, class 3
Calculated Molecular Weight 887 aa, 100 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC053651
Gene Symbol VPS34
Gene ID (NCBI) 5289
RRIDAB_10646474
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3C3(Phosphatidylinositol 3-kinase catalytic subunit type 3) is also named as VPS34 and belongs to the PI3/PI4-kinase family.It is a catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes.It can form a dimer(PMID:20339072).

Protocols

Product Specific Protocols
WB protocol for VPS34 antibody 13723-1-APDownload protocol
IHC protocol for VPS34 antibody 13723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Pharmacol

Integrated Network Pharmacology and Lipidomics to Reveal the Inhibitory Effect of Qingfei Oral Liquid on Excessive Autophagy in RSV-Induced Lung Inflammation.

Authors - Lili Lin
ratWB

J Cardiovasc Transl Res

The Protective Mechanism of Dexmedetomidine in Regulating Atg14L-Beclin1-Vps34 Complex Against Myocardial Ischemia-Reperfusion Injury.

Authors - Yanna Li
humanWB

Nat Struct Mol Biol

Reduction of DHHC5-mediated beclin 1 S-palmitoylation underlies autophagy decline in aging

Authors - Rui Guo