VPS34 Polyclonal antibody

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  WB analysis of Jurkat using 13723-1-AP

  WB result of VPS34 antibody (13723-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS34 transfected Jurkat cells.

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  WB analysis of HeLa using 13723-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13723-1-AP (VPS34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13723-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13723-1-AP (VPS34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13723-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13723-1-AP (VPS34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 13723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13723-1-AP (VPS34 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 13723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13723-1-AP (VPS34 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

VPS34 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13723-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Synonyms

PIK3C3, EC:2.7.1.137, hVps34, PI 3 Kinase Class 3, PI 3-kinase p100

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue, mouse lung tissue, Jurkat cells
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

13723-1-AP targets VPS34 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phosphoinositide-3-kinase, class 3
• Calculated Molecular Weight: 887 aa, 100 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC053651
• Gene Symbol: VPS34
• Gene ID (NCBI): 5289
• RRID: AB_10646474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NEB9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3C3(Phosphatidylinositol 3-kinase catalytic subunit type 3) is also named as VPS34 and belongs to the PI3/PI4-kinase family.It is a catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes.It can form a dimer(PMID:20339072).

Publications

Species	Application	Title
pig	WB	J Virol; Potassium molybdate blocks APN-dependent coronavirus entry by degrading receptor via PIK3C3-mediated autophagy; Authors - Yunhang Zhang ; KD Validated
mouse	WB	Front Pharmacol; Integrated Network Pharmacology and Lipidomics to Reveal the Inhibitory Effect of Qingfei Oral Liquid on Excessive Autophagy in RSV-Induced Lung Inflammation.; Authors - Lili Lin
rat	WB	J Cardiovasc Transl Res; The Protective Mechanism of Dexmedetomidine in Regulating Atg14L-Beclin1-Vps34 Complex Against Myocardial Ischemia-Reperfusion Injury.; Authors - Yanna Li
human	WB	Nat Struct Mol Biol; Reduction of DHHC5-mediated beclin 1 S-palmitoylation underlies autophagy decline in aging; Authors - Rui Guo