PI3K p101 Polyclonal antibody

PI3K p101 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 13329-1-AP

Synonyms

F730038I15Rik, FOAP 2, p101, P101 PI3K, PI3 kinase p101 subunit, PI3K p101, PIK3R5, Protein FOAP 2, PtdIns 3 kinase p101

---

Validation Data Gallery

• 

  WB analysis of rat lymph using 13329-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 13329-1-AP (PI3K p101 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 13329-1-AP

  Immunofluorescent analysis of HepG2 cells, using PIK3R5 antibody 13329-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IHC staining of human ovary tumor using 13329-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13329-1-AP (PI3K p101 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 13329-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13329-1-AP (PI3K p101 antibody at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: rat lymph tissue
• Positive IHC detected in: human ovary tumor tissue, human liver cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 5 publications below

Product Information

13329-1-AP targets PI3K p101 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PI3K p101 fusion protein Ag4137
• Full Name: phosphoinositide-3-kinase, regulatory subunit 5
• Calculated molecular weight: 880 aa, 97 kDa
• Observed molecular weight: 55 kDa
• GenBank accession number: BC028212
• Gene symbol: PIK3R5
• Gene ID (NCBI): 23533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	World J Gastrointest Oncol; Qingjie Fuzheng granules inhibit colorectal cancer cell growth by the PI3K/AKT and ERK pathways.; Authors - Hong Yang
human	WB	Exp Ther Med; Scutellaria barbata D. Don inhibits migration and invasion of colorectal cancer cells via suppression of PI3K/AKT and TGF-β/Smad signaling pathways.; Authors - Yiyi Jin
human	WB	Chin Med; Shikonin and 4-hydroxytamoxifen synergistically inhibit the proliferation of breast cancer cells through activating apoptosis signaling pathway in vitro and in vivo.; Authors - Hong-Yan Lin
mouse	WB	Cell Tissue Res; Lycium barbarum polysaccharide attenuates diabetic testicular dysfunction via inhibition of the PI3K/Akt pathway-mediated abnormal autophagy in male mice.; Authors - Guang-Jiang Shi
mouse	WB	Allergy; miRNA-155 controls mast cell activation by regulating the PI3Kγ pathway and anaphylaxis in a mouse model.; Authors - K Biethahn