PI3K p101 Polyclonal antibody

PI3K p101 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13329-1-AP

Synonyms

F730038I15Rik, FOAP 2, p101, P101 PI3K, PI3 kinase p101 subunit, PI3K p101, PIK3R5, Protein FOAP 2, PtdIns 3 kinase p101



Tested Applications

Positive WB detected inrat lymph tissue
Positive IHC detected inhuman ovary tumor tissue, human liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

13329-1-AP targets PI3K p101 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PI3K p101 fusion protein Ag4137
Full Name phosphoinositide-3-kinase, regulatory subunit 5
Calculated molecular weight 880 aa, 97 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC028212
Gene symbol PIK3R5
Gene ID (NCBI) 23533
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PI3K p101 antibody 13329-1-APDownload protocol
IHC protocol for PI3K p101 antibody 13329-1-APDownload protocol
IF protocol for PI3K p101 antibody 13329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

World J Gastrointest Oncol

Qingjie Fuzheng granules inhibit colorectal cancer cell growth by the PI3K/AKT and ERK pathways.

Authors - Hong Yang
humanWB

Exp Ther Med

Scutellaria barbata D. Don inhibits migration and invasion of colorectal cancer cells via suppression of PI3K/AKT and TGF-β/Smad signaling pathways.

Authors - Yiyi Jin
humanWB

Chin Med

Shikonin and 4-hydroxytamoxifen synergistically inhibit the proliferation of breast cancer cells through activating apoptosis signaling pathway in vitro and in vivo.

Authors - Hong-Yan Lin
mouseWB

Cell Tissue Res

Lycium barbarum polysaccharide attenuates diabetic testicular dysfunction via inhibition of the PI3K/Akt pathway-mediated abnormal autophagy in male mice.

Authors - Guang-Jiang Shi
mouseWB

Allergy

miRNA-155 controls mast cell activation by regulating the PI3Kγ pathway and anaphylaxis in a mouse model.

Authors - K Biethahn