PINX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PINX1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 12368-1-AP

Synonyms

Pin2-interacting protein X1, Pin2 interacting protein X1, PIN2 interacting protein 1, LPTS, LPTL

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Validation Data Gallery

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  WB analysis using 12368-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 12368-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12368-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 12368-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 12368-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12368-1-AP (PINX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, A431 cells, C6 cells, NIH/3T3 cells, HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 8 publications below

Product Information

12368-1-AP targets PINX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PINX1 fusion protein Ag3043
• Full Name: PIN2-interacting protein 1
• Calculated Molecular Weight: 328 aa, 37 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC015479
• Gene Symbol: PINX1
• Gene ID (NCBI): 54984
• RRID: AB_2164405
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PINX1, also named as LPTL, LPTS and Protein 67-11-3, is a Liver-related putative tumor suppressor. It belongs to the PINX1 family. PINX1 is a microtubule-binding protein essential for faithful chromosome segregation. It mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. PINX1 inhibits telomerase activity. It may inhibit cell proliferation and act as tumor suppressor. PINX1 has been identified as a critical telomerase inhibitor and proposed to be a putative tumor suppressor gene. Loss of PinX1 has been found in a large variety of malignancies, but the expression status in epithelial ovarian tumors has not been investigated.

Publications

Species	Application	Title
human	WB	Oncogene; Anthracyclines disrupt telomere maintenance by telomerase through inducing PinX1 ubiquitination and degradation.; Authors - Zhang B B ; KD Validated
human	IHC	Mol Cancer; The depletion of PinX1 involved in the tumorigenesis of non-small cell lung cancer promotes cell proliferation via p15/cyclin D1 pathway.; Authors - Xiao-Peng Tian
human	WB,IHC	Am J Cancer Res; PINX1 promotes malignant transformation of thyroid cancer through the activation of the AKT/MAPK/β-catenin signaling pathway.; Authors - JiHoon Kang ; KD Validated
human	WB,IHC	Mol Cancer; PinX1 suppresses bladder urothelial carcinoma cell proliferation via the inhibition of telomerase activity and p16/cyclin D1 pathway.; Authors - Liu Jian-Ye JY
human	WB,IHC	Cancer Lett; PMID: 25045845; Authors - Xiao-Peng Tian
human	WB,IHC	Cancer Sci; Decreased expression of PinX1 protein is correlated with tumor development and is a new independent poor prognostic factor in ovarian carcinoma.; Authors - Cai Mu-Yan MY