PIP4K2B Polyclonal antibody

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  WB analysis of HeLa using 13218-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13218-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13218-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13218-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13218-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13218-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 13218-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13218-1-AP (PIP4K2B antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas cancer using 13218-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13218-1-AP (PIP4K2B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 13218-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13218-1-AP (PIP4K2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13218-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13218-1-AP (PIP4K2B Antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

PIP4K2B Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13218-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

PI(5)P 4 kinase type II beta, PI5P4KB, PIP4K2B, PIP4KII beta, PIP5K2B, PIP5KIIB, PIP5KIIbeta

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells
• Positive IHC detected in: human pancreas cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

13218-1-AP targets PIP4K2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4099

  Product name: Recombinant human PIP4K2B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-281 aa of BC027459

  Sequence: MSSNCTSTTAVAVAPLSASKTKTKKKHFVCQKVKLFRASEPILSVLMWGVNHTINELSNVPVPVMLMPDDFKAYSKIKVDNHLFNKENLPSRFKFKEYCPMVFRNLRERFGIDDQDYQNSVTRSAPINSDSQGRCGTRFLTTYDRRFVIKTVSSEDVAEMHNILKKYHQFIVECHGNTLLPQFLGMYRLTVDGVETYMVVTRNVFSHRLTVHRKYDLKGSTVAREASDKEKAKDLPTFKDNDFLNEGQKLHVGEESKKNFLEKLKRDVEEILVLSPGRRIA

• Full Name: phosphatidylinositol-5-phosphate 4-kinase, type II, beta
• Calculated Molecular Weight: 416 aa, 47 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC027459
• Gene Symbol: PIP4K2B
• Gene ID (NCBI): 8396
• RRID: AB_10644482
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P78356
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIP4K2B, also named as PIP5K2B, participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. PIP4K2B has a homodimer. It binds TNFRSF1A(24/32/50kd).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Commun; MANF regulates hypothalamic control of food intake and body weight.; Authors - Su Yang ; KD Validated
mouse	IHC	Dev Cell; PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria interplay.; Authors - Archna Ravi
human	WB	JBMR Plus; Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament; Authors - Hideaki Nakajima
human	WB	Eur J Med Chem; Design, synthesis and biological evaluation of the activity-based probes for FGFR covalent inhibitor; Authors - Dandan Zhu