PIP4K2B Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83647-3-RR (same clone as 83647-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83647-3-RR (PIP4K2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83647-3-RR (same clone as 83647-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83647-3-RR (PIP4K2B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 83647-3-RR (same clone as 83647-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83647-3-RR (PIP4K2B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 83647-3-RR (same clone as 83647-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PIP4K2B antibody (83647-3-RR, Clone: 240645F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83647-3-RR (same clone as 83647-3-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug PIP4K2B Recombinant antibody (83647-3-RR, Clone:240645F10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

PIP4K2B Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Cat No. 83647-3-PBS

Clone No.240645F10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Synonyms

Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, EC:2.7.1.149, Diphosphoinositide kinase 2-beta, 240645F10

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Product Information

83647-3-PBS targets PIP4K2B in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4099

  Product name: Recombinant human PIP4K2B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-281 aa of BC027459

  Sequence: MSSNCTSTTAVAVAPLSASKTKTKKKHFVCQKVKLFRASEPILSVLMWGVNHTINELSNVPVPVMLMPDDFKAYSKIKVDNHLFNKENLPSRFKFKEYCPMVFRNLRERFGIDDQDYQNSVTRSAPINSDSQGRCGTRFLTTYDRRFVIKTVSSEDVAEMHNILKKYHQFIVECHGNTLLPQFLGMYRLTVDGVETYMVVTRNVFSHRLTVHRKYDLKGSTVAREASDKEKAKDLPTFKDNDFLNEGQKLHVGEESKKNFLEKLKRDVEEILVLSPGRRIA

• Full Name: phosphatidylinositol-5-phosphate 4-kinase, type II, beta
• Calculated Molecular Weight: 416 aa, 47 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC027459
• Gene Symbol: PIP4K2B
• Gene ID (NCBI): 8396
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P78356
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PIP4K2B, also named as PIP5K2B, participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. PIP4K2B has a homodimer.