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  • KD/KO Validated

PIP4K2C Polyclonal antibody

PIP4K2C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17077-1-AP

Synonyms

PI(5)P 4 kinase type II gamma, PIP4K2C, PIP4KII gamma, PIP5K2C



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, PC-12 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17077-1-AP targets PIP4K2C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIP4K2C fusion protein Ag10989
Full Name phosphatidylinositol-5-phosphate 4-kinase, type II, gamma
Calculated Molecular Weight 421 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC028596
Gene Symbol PIP4K2C
Gene ID (NCBI) 79837
RRIDAB_2715526
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIP4K2C is involved in several processes, including 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process; negative regulation of 1-phosphatidylinositol-4-phosphate 5-kinase activity; and positive regulation of autophagosome assembly. PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism.

Protocols

Product Specific Protocols
WB protocol for PIP4K2C antibody 17077-1-APDownload protocol
IHC protocol for PIP4K2C antibody 17077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

Inhibition of PIP4Kγ ameliorates the pathological effects of mutant huntingtin protein.

Authors - Ismael Al-Ramahi
  • KD Validated
humanWB

Cell Rep

PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent Mechanism.

Authors - Diana G Wang
  • KO Validated
humanWB

Autophagy Rep

Upregulation of the ESCRT pathway and multivesicular bodies accelerates degradation of proteins associated with neurodegeneration

Authors - Ron Benyair