PIP4K2C Polyclonal antibody

• Featured Product
• KD/KO Validated

PIP4K2C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17077-1-AP

Synonyms

PI(5)P 4 kinase type II gamma, PIP4K2C, PIP4KII gamma, PIP5K2C

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Validation Data Gallery

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  WB analysis using 17077-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17077-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 17077-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17077-1-AP (PIP4K2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17077-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17077-1-AP (PIP4K2C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, PC-12 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below

Product Information

17077-1-AP targets PIP4K2C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIP4K2C fusion protein Ag10989
• Full Name: phosphatidylinositol-5-phosphate 4-kinase, type II, gamma
• Calculated Molecular Weight: 421 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC028596
• Gene Symbol: PIP4K2C
• Gene ID (NCBI): 79837
• RRID: AB_2715526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIP4K2C is involved in several processes, including 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process; negative regulation of 1-phosphatidylinositol-4-phosphate 5-kinase activity; and positive regulation of autophagosome assembly. PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism.

Publications

Species	Application	Title
human	WB	Elife; Inhibition of PIP4Kγ ameliorates the pathological effects of mutant huntingtin protein.; Authors - Ismael Al-Ramahi ; KD Validated
human	WB	Cell Rep; PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent Mechanism.; Authors - Diana G Wang ; KO Validated
human	WB	Autophagy Rep; Upregulation of the ESCRT pathway and multivesicular bodies accelerates degradation of proteins associated with neurodegeneration; Authors - Ron Benyair