PIP5K1A Monoclonal antibody

PIP5K1A Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3E5G12

Cat no : 67483-1-Ig

Synonyms

PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha

---

Validation Data Gallery

• 

  WB analysis using 67483-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis using 67483-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67483-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 67483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67483-1-Ig (PIP5K1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67483-1-Ig (PIP5K1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, Hela cell, HepG2 cells, L02 cells, K-562 cells, Jurkat cells, MOLT-4 cells, HeLa cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67483-1-Ig targets PIP5K1A in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PIP5K1A fusion protein Ag8355
• Full Name: phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
• Calculated Molecular Weight: 56 kDa, 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC007833
• Gene Symbol: PIP5K1A
• Gene ID (NCBI): 8394
• RRID: AB_2882710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa.

Publications

Species	Application	Title
human	WB	J Virol; The Rac1-PAK1-Arp2/3 signaling axis regulates CHIKV nsP1-induced filopodia and optimal viral genome replication; Authors - Olivier Aïqui-Reboul-Paviet