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  • KD/KO Validated

PIP5K1B Polyclonal antibody

PIP5K1B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12541-1-AP

Synonyms

PIP5K1 beta, PIP5K1B, PIP5KIbeta, Protein STM 7, PtdIns(4)P 5 kinase 1 beta, STM7



Tested Applications

Positive WB detected inhuman testis tissue, human brain tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman lung tissue, human heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12541-1-AP targets PIP5K1B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIP5K1B fusion protein Ag3261
Full Name phosphatidylinositol-4-phosphate 5-kinase, type I, beta
Calculated Molecular Weight 549 aa, 62 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC030587
Gene Symbol PIP5K1B
Gene ID (NCBI) 8395
RRIDAB_10734598
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PIP5K1B antibody 12541-1-APDownload protocol
IHC protocol for PIP5K1B antibody 12541-1-APDownload protocol
IP protocol for PIP5K1B antibody 12541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Invest

Spastic paraplegia proteins spastizin and spatacsin mediate autophagic lysosome reformation.

Authors - Jaerak Chang
mouseWB

EMBO J

PTPRN2 and PLCβ1 promote metastatic breast cancer cell migration through PI(4,5)P2-dependent actin remodeling.

Authors - Caitlin A Sengelaub
humanWB

EMBO J

Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKHM1 membrane cycling during autophagosome-lysosome fusion.

Authors - Takashi Baba
  • KD Validated
humanWB

Cell Chem Biol

Targeting the PI5P4K Lipid Kinase Family in Cancer Using Covalent Inhibitors.

Authors - Sindhu Carmen Sivakumaren

EMBO J

Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKMH1 membrane cycling during autophagosome-lysosome fusion.

Authors - Takashi Baba