PIP5K3 Polyclonal antibody

• Featured Product
• KD/KO Validated

PIP5K3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA, Dot Blot

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 13361-1-AP

Synonyms

CFD, FAB1, KIAA0981, PIKFYVE, PIP5K, PIP5K3, PIPkin III, Type III PIP kinase

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Validation Data Gallery

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  WB analysis of mouse lung using 13361-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 13361-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13361-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13361-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13361-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13361-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse lung tissue, Jurkat cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

13361-1-AP targets PIP5K3 in WB, IHC, ELISA, Dot Blot applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIP5K3 fusion protein Ag4177
• Full Name: phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III
• Calculated Molecular Weight: 451 aa, 50 kDa, 237 kDa
• Observed Molecular Weight: 237 kDa
• GenBank Accession Number: BC032389
• Gene Symbol: PIP5K3
• Gene ID (NCBI): 200576
• RRID: AB_10638310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIP5K3 (1-phosphatidylinositol 3-phosphate 5-kinase), also callled PIKFYVE, belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol (PMID: 15902656; 9858586). The content of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) in endosomal membranes changes dynamically with fission and fusion events that generate or absorb intracellular transport vesicles. PIKFYVE is the PtdIns(3,5)P2-producing component of a trimolecular complex that tightly regulates the level of PtdIns(3,5)P2. Other components of this complex are the PIKFYVE activator VAC14 and the PtdIns(3,5)P2 phosphatase FIG4 (PMID: 17556371). In addition to its phosphoinositide 5-kinase activity, PIKFYVE also has protein kinase activity (PubMed: 11714711; 17556371).PIP5K3 has four isoforms with molecular weights of 237 kDa, 50 kDa, 51 kDa and 62 kDa. This antibody primarily recognizes the 237 kDa isoform.

Publications

Species	Application	Title
		Mol Cell; TSC1 binding to lysosomal PIPs is required for TSC complex translocation and mTORC1 regulation.; Authors - Katharina Fitzian ; KD Validated
human	WB	Elife; Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes.; Authors - Sai Srinivas Panapakkam Giridharan
pig	WB	PLoS Pathog; Pseudorabies virus inhibits progesterone-induced inactivation of TRPML1 to facilitate viral entry; Authors - Bing-Qian Su
human	IHC	Front Oncol; TRIM68, PIKFYVE, and DYNLL2: The Possible Novel Autophagy- and Immunity-Associated Gene Biomarkers for Osteosarcoma Prognosis.; Authors - Jie Jiang
		Eur J Pharmacol; Inhibition of c-Rel DNA binding is critical for the anti-inflammatory effects of novel PIKfyve inhibitor.; Authors - Masaomi Terajima
mouse	Dot Blot	BMC Immunol; miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment.; Authors - Jingsong Cao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kincaid (Verified Customer) (05-21-2024)

This antibody works very well. I use it 1:200 - overnight incubation at 4°C (3% BSA in TBST). I have validated with H4 cell lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: H4 cell

PIP5K3 Antibody Western Blot validation (1:200 dilution) in H4 cell (Cat no:13361-1-AP)