PIP5KL1 Polyclonal antibody

PIP5KL1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 17547-1-AP

Synonyms

PI(4)P 5 kinase like protein 1, PIP5KL1, PIPKH

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Validation Data Gallery

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  WB analysis of HEK-293 using 17547-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17547-1-AP (PIP5KL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17547-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17547-1-AP (PIP5KL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 17547-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 17547-1-AP (PIP5KL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human brain tissue
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17547-1-AP targets PIP5KL1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIP5KL1 fusion protein Ag11653
• Full Name: phosphatidylinositol-4-phosphate 5-kinase-like 1
• Calculated Molecular Weight: 22 kDa, 44 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC042184
• Gene Symbol: PIP5KL1
• Gene ID (NCBI): 138429
• RRID: AB_2252440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.