PIPPIN Polyclonal antibody

PIPPIN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25013-1-AP

Synonyms

CSDC2, RNA-binding protein PIPPin, RNA binding protein PIPPin, Cold shock domain-containing protein C2

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Validation Data Gallery

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  WB analysis of mouse brain using 25013-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25013-1-AP (PIPPIN antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 25013-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25013-1-AP (PIPPIN antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 25013-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25013-1-AP (PIPPIN antibody at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25013-1-AP targets PIPPIN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIPPIN fusion protein Ag16216
• Full Name: cold shock domain containing C2, RNA binding
• Calculated Molecular Weight: 153 aa, 17 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC067113
• Gene Symbol: CSDC2
• Gene ID (NCBI): 27254
• RRID: AB_2879845
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIPPIN, also known as CSDC2 (Cold shock domain-containing protein C2) is an RNA‐binding protein (RBP), highly enriched in the rat brain, specifically enriched in some pyramidal neurons of the cerebral cortex and in the Purkinje cells of the cerebellum (PMID: 10446180). PIPPIN has the potential to undergo different posttranslational modifications and might be a good candidate to regulate the synthesis of specific proteins in response to extracellular stimuli. Nuclear CSDC2 is connected to proliferation and cytoplasmic CSDC2 to terminal differentiation in the decidua and that CSDC2 could regulate differentiation during decidua development (PMID: 30078185, PMID: 17053029).