PIR Polyclonal antibody

• Featured Product
• KD/KO Validated

PIR Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10263-1-AP

Synonyms

Probable quercetinase, Probable quercetin 2,3-dioxygenase PIR, Pirin, EC:1.13.11.24

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Validation Data Gallery

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  WB analysis of HepG2 using 10263-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10263-1-AP (PIR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10263-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10263-1-AP (PIR antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10263-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10263-1-AP (PIR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10263-1-AP

  IP result of anti-PIR (IP:10263-1-AP, 4ug; Detection:10263-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IHC staining of human stomach using 10263-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10263-1-AP (PIR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

10263-1-AP targets PIR in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIR fusion protein Ag0330
• Full Name: pirin (iron-binding nuclear protein)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC002517
• Gene Symbol: PIR
• Gene ID (NCBI): 8544
• RRID: AB_2283879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pirin (iron-binding nuclear protein, PIR) is a highly conserved 32-kDa protein consisting of 290 amino acids. Pirin mRNA is expressed weakly in all human tissues. Confocal immunofluorescence experiments demonstrate a predominant localization of Pirin within dot-like subnuclear structures. Pirin interacts with the oncoprotein B-cell lymphoma 3-encoded (Bcl-3) and nuclear factor I (NFI).

Publications

Species	Application	Title
human	WB	Cell Cycle; PIR promotes tumorigenesis of breast cancer by upregulating cell cycle activator E2F1.; Authors - Muhammad Suleman ; KD Validated