PIR Monoclonal antibody
PIR Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2A10E4
Cat no : 67516-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HSC-T6 cells, HeLa cells, K-562 cells, NIH/3T3 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67516-1-Ig targets PIR in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PIR fusion protein Ag29969 |
Full Name | pirin (iron-binding nuclear protein) |
Calculated Molecular Weight | 32 kDa |
Observed Molecular Weight | 32 kDa |
GenBank Accession Number | BC002517 |
Gene Symbol | PIR |
Gene ID (NCBI) | 8544 |
RRID | AB_2882737 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Pirin (iron-binding nuclear protein, PIR) is a highly conserved 32-kDa protein consisting of 290 amino acids. Pirin mRNA is expressed weakly in all human tissues. Confocal immunofluorescence experiments demonstrate a predominant localization of Pirin within dot-like subnuclear structures. Pirin interacts with the oncoprotein B-cell lymphoma 3-encoded (Bcl-3) and nuclear factor I (NFI).
Protocols
Product Specific Protocols | |
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WB protocol for PIR antibody 67516-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |