PIRT Polyclonal antibody

• 

  WB analysis of SH-SY5Y using 20990-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20990-1-AP (PIRT Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

PIRT Polyclonal Antibody for WB, ELISA

Cat No. 20990-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Synonyms

hCG_1776018, PIRT, TRPV1 complex

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Tested Applications

• Positive WB detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

20990-1-AP targets PIRT in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: phosphoinositide-interacting regulator of transient receptor potential channels
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_001101387
• Gene Symbol: PIRT
• Gene ID (NCBI): 644139
• RRID: AB_2878785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P0C851
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIRT is a key component of the TRPV1 complex that positively regulates TRPV1 activity. It binds various phosphoinositide, including phosphatidylinositol 4,5-bisphosphate (PIP2), but not phosphatidylinositol (PI). PIRT is a membrane protein with MW 15-25 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	EMBO Rep; Defective branched-chain amino acid catabolism in dorsal root ganglia contributes to mechanical pain; Authors - Huijing Xie
mouse	IF	Evid Based Complement Alternat Med; C-Phycocyanin Alleviates Bladder Inflammation and Dysfunction in Cyclophosphamide-Induced Cystitis in a Mouse Model by Inhibiting COX-2 and EP4.; Authors - Xing-Qi Bao