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  • KD/KO Validated

PISD Polyclonal antibody

PISD Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16401-1-AP

Synonyms

PSSC, PSDC, Phosphatidylserine decarboxylase proenzyme, mitochondrial, Phosphatidylserine decarboxylase beta chain, Phosphatidylserine decarboxylase alpha chain



Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, HepG2 cells, human kidney tissue, human spleen tissue, K-562 cells, L02 cells, NIH/3T3 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16401-1-AP targets PISD in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PISD fusion protein Ag9499
Full Name phosphatidylserine decarboxylase
Calculated Molecular Weight375aa,43 kDa; 408aa,46 kDa
Observed Molecular Weight 45~55 kDa
GenBank Accession NumberBC001482
Gene Symbol PISD
Gene ID (NCBI) 23761
RRIDAB_2186673
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.This antibody is specific to PISD. The sequence correspond to the beta isoform.

Protocols

Product Specific Protocols
WB protocol for PISD antibody 16401-1-APDownload protocol
IHC protocol for PISD antibody 16401-1-APDownload protocol
IP protocol for PISD antibody 16401-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Aberrant ER-mitochondria communication is a common pathomechanism in mitochondrial disease

Authors - Patricia Morcillo
humanWB

Cancer Lett

LACTB suppresses melanoma progression by attenuating PP1A and YAP interaction.

Authors - Yawen Ma
human

Front Cell Dev Biol

Screening and diagnosis of triple negative breast cancer based on rapid metabolic fingerprinting by conductive polymer spray ionization mass spectrometry and machine learning

Authors - Yaoyao Song
humanWB

J Clin Biochem Nutr

Metabolic changes induced by TGF-β1 via reduced expression of phosphatidylserine decarboxylase during myofibroblast transition

Authors - Nobuhiko Uchida
humanWB

Cell Stem Cell

The Mitochondrial Transacylase, Tafazzin, Regulates for AML Stemness by Modulating Intracellular Levels of Phospholipids.

Authors - Ayesh K Seneviratne
  • KO Validated