PISD Polyclonal antibody

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• PISD Polyclonal antibody (16401-1-AP)

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PISD Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16401-1-AP Copied!

Publications (11)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

PSSC, PSDC, Phosphatidylserine decarboxylase proenzyme, mitochondrial, Phosphatidylserine decarboxylase beta chain, Phosphatidylserine decarboxylase alpha chain

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  WB analysis of K-562 using 16401-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 16401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 16401-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of L02 using 16401-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of L02 using 16401-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of L02 using 16401-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human spleen using 16401-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human kidney using 16401-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of COLO 320 using 16401-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 16401-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 16401-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of L02 using 16401-1-AP

  IP result of anti-PISD (IP:16401-1-AP, 3ug; Detection:16401-1-AP 1:500) with L02 cells lysate 1400ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 16401-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16401-1-AP (PISD antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 16401-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16401-1-AP (PISD antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, COLO 320 cells, HepG2 cells, human kidney tissue, human spleen tissue, K-562 cells, L02 cells, NIH/3T3 cells
• Positive IP detected in: L02 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16401-1-AP targets PISD in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9499

  Product name: Recombinant human PISD protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-375 aa of BC001482

  Sequence: MMCQSEARQGPELRAAKWLHFPQLALRRRLGQLSCMSRPALKLRSWPLTVLYYLLPFGALRPLSRVGWRPVSRVALYKSVPTRLLSRAWGRLNQVELPHWLRRPVYSLYIWTFGVNMKEAAVEDLHHYRNLSEFFRRKLKPQARPVCGLHSVISPSDGRILNFGQVKNCEVEQVKGVTYSLESFLGPRMCTEDLPFPPAASCDSFKNQLVTREGNELYHCVIYLAPGDYHCFHSPTDWTVSHRRHFPGSLMSVNPGMARWIKELFCHNERVVLTGDWKHGFFSLTAVGATNVGSIRIYFDRDLHTNSPRHSKGSYNDFSFVTHTNREGVPMRKGEHLGEFNLGSTIVLIFEAPKDFNFQLKTGQKIRFGEALGSL

• Full Name: phosphatidylserine decarboxylase
• Calculated Molecular Weight: 375aa,43 kDa; 408aa,46 kDa
• Observed Molecular Weight: 45~55 kDa
• GenBank Accession Number: BC001482
• Gene Symbol: PISD
• Gene ID (NCBI): 23761
• RRID: AB_2186673
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UG56
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.This antibody is specific to PISD. The sequence correspond to the beta isoform.

Publications

What published studies show

The PISD Polyclonal antibody (Cat# 16401-1-AP) has 11 citations published in journals including Cell Stem Cell, Cell Death & Disease, Cancer Letters, Journal of Cell Biology, Science Advances, Cell Molecular Gastroenterology and Hepatology, Biomolecules, Frontiers in Cell and Developmental Biology, Journal of Animal Science and Biotechnology, Journal of Clinical Biochemistry and Nutrition, and bioRxiv. Research fields span mitochondrial biology, lipid metabolism, cancer, liver disease, neurobiology, and autophagy.

Automatically generated from the 11 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Stem Cell; The Mitochondrial Transacylase, Tafazzin, Regulates for AML Stemness by Modulating Intracellular Levels of Phospholipids.; Authors - Ayesh K Seneviratne ; KO Validated
human	WB	Cell Death Dis; Aberrant ER-mitochondria communication is a common pathomechanism in mitochondrial disease; Authors - Patricia Morcillo
human	WB	Cancer Lett; LACTB suppresses melanoma progression by attenuating PP1A and YAP interaction.; Authors - Yawen Ma
mouse,human	WB	Cell Mol Gastroenterol Hepatol; Overlapping Yet Distinct Functions of Vacuole Membrane Protein 1 and Transmembrane Protein 41B in Modulating Hepatic Lipoprotein Secretion and Autophagy.; Authors - Allen Chen ; KO Validated
		J Anim Sci Biotechnol; Se-enriched yeast improves meat quality through glycerophospholipid metabolism in finishing pigs: insights from a multi-omics analysis.; Authors - Xiangqi Qiu
human	WB,IF	J Cell Biol; PISD acts as a switch between lipophagy and fatty acid transfer to mitochondria; Authors - Hanbing Xue ; KD Validated