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PISD Polyclonal antibody
PISD Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
Cat no : 16401-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, COLO 320 cells, HepG2 cells, human kidney tissue, human spleen tissue, K-562 cells, L02 cells, NIH/3T3 cells |
Positive IP detected in | L02 cells |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 4 publications below |
Product Information
16401-1-AP targets PISD in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PISD fusion protein Ag9499 |
Full Name | phosphatidylserine decarboxylase |
Calculated Molecular Weight | 375aa,43 kDa; 408aa,46 kDa |
Observed Molecular Weight | 45~55 kDa |
GenBank Accession Number | BC001482 |
Gene Symbol | PISD |
Gene ID (NCBI) | 23761 |
RRID | AB_2186673 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.This antibody is specific to PISD. The sequence correspond to the beta isoform.
Protocols
Product Specific Protocols | |
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WB protocol for PISD antibody 16401-1-AP | Download protocol |
IHC protocol for PISD antibody 16401-1-AP | Download protocol |
IP protocol for PISD antibody 16401-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Dis Aberrant ER-mitochondria communication is a common pathomechanism in mitochondrial disease | ||
Cancer Lett LACTB suppresses melanoma progression by attenuating PP1A and YAP interaction. | ||
Front Cell Dev Biol Screening and diagnosis of triple negative breast cancer based on rapid metabolic fingerprinting by conductive polymer spray ionization mass spectrometry and machine learning | ||
J Clin Biochem Nutr Metabolic changes induced by TGF-β1 via reduced expression of phosphatidylserine decarboxylase during myofibroblast transition | ||
Cell Stem Cell The Mitochondrial Transacylase, Tafazzin, Regulates for AML Stemness by Modulating Intracellular Levels of Phospholipids.
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