Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human lung tissue, human brain tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16613-1-AP targets PITPNA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9849 Product name: Recombinant human PITPNA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-270 aa of BC045108 Sequence: MVLLKEYRVILPVSVDEYQVGQLYSVAEASKNETGGGEGVEVLVNEPYEKDGEKGQYTHKIYHLQSKVPTFVRMLAPEGALNIHEKAWNAYPYCRTVITNEYMKEDFLIKIETWHKPDLGTQENVHKLEPEAWKHVEAVYIDIADRSQVLSKDYKAEEDPAKFKSIKTGRGPLGPNWKQELVNQKDCPYMCAYKLVTVKFKWWGLQNKVENFIHKQERRLFTNFHRQLFCWLDKWVDLTMDDIRRMEEETKRQLDEMRQKDPVKGMTADD Predict reactive species |
| Full Name | phosphatidylinositol transfer protein, alpha |
| Calculated Molecular Weight | 270 aa, 32 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC045108 |
| Gene Symbol | PITPNA |
| Gene ID (NCBI) | 5306 |
| RRID | AB_2299692 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q00169 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PITPNA antibody 16613-1-AP | Download protocol |
| IP protocol for PITPNA antibody 16613-1-AP | Download protocol |
| WB protocol for PITPNA antibody 16613-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PITPNA polyclonal antibody (Cat# 16613-1-AP) has 3 total citations published in Nature Communications, Developmental Cell, and BMC Complementary and Alternative Medicine. The associated research fields include pancreatic beta-cell dysfunction in type 2 diabetes, Golgi lipid signaling and cell polarity during neurogenesis, and differential proteomics of neuropathic pain treatment via electroacupuncture. Cited applications include Western blot and immunofluorescence in mouse and rat models.
| Species | Application | Title |
|---|---|---|
Nat Commun Restoration of PITPNA in Type 2 diabetic human islets reverses pancreatic beta-cell dysfunction | ||
Dev Cell A Golgi Lipid Signaling Pathway Controls Apical Golgi Distribution and Cell Polarity during Neurogenesis. | ||
BMC Complement Altern Med Differential proteomics analysis of the analgesic effect of electroacupuncture intervention in the hippocampus following neuropathic pain in rats. |
