PITPNB Polyclonal antibody

• Featured Product
• KD/KO Validated

PITPNB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13110-1-AP

Synonyms

PI TP beta, PITPNB, PtdIns transfer protein beta, PtdInsTP, PtdInsTP beta, VIB1B

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Validation Data Gallery

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  WB analysis of mouse lung using 13110-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13110-1-AP (PITPNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13110-1-AP (PITPNB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 13110-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13110-1-AP (PITPNB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13110-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13110-1-AP (PITPNB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13110-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13110-1-AP (PITPNB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 13110-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13110-1-AP (PITPNB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13110-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13110-1-AP (PITPNB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13110-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13110-1-AP (PITPNB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse lung tissue, HeLa cells, Raji cells, rat lung tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below

Product Information

13110-1-AP targets PITPNB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PITPNB fusion protein Ag3774
• Full Name: phosphatidylinositol transfer protein, beta
• Calculated Molecular Weight: 272 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC031427
• Gene Symbol: PITPNB
• Gene ID (NCBI): 23760
• RRID: AB_2164999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Virol; Nir2 Is an Effector of VAPs Necessary for Efficient Hepatitis C Virus Replication and Phosphatidylinositol 4-Phosphate Enrichment at the Viral Replication Organelle.; Authors - Hongliang Wang ; KO Validated
human	WB	Elife; The ORP9-ORP11 dimer promotes sphingomyelin synthesis; Authors - Birol Cabukusta ; KO Validated