PJA1 Recombinant monoclonal antibody

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• PJA1 Recombinant monoclonal antibody (86892-1-RR)

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  WB analysis using 86892-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86892-1-RR (PJA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86892-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86892-1-RR against Human PJA1 were performed. The affinity constant is 11.6 nM.

PJA1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86892-1-RR

Clone No.251875A3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

E3 ubiquitin-protein ligase Praja-1, EC:2.3.2.27, praja ring finger 1, Praja1, RING finger protein 70

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Tested Applications

• Positive WB detected in: RT-4 cells, K-562 cells, rat brain tisuue, mouse cerebellum tissue, rat cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86892-1-RR targets PJA1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag11820

  Product name: Recombinant human PJA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 294-643 aa of BC075803

  Sequence: ANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL

• Full Name: praja ring finger 1
• Calculated Molecular Weight: 643 aa, 71 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC075803
• Gene Symbol: PJA1
• Gene ID (NCBI): 64219
• RRID: AB_3745191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NG27
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PJA1(E3 ubiquitin-protein ligase Praja-1), also named as RNF70, regulates the level of PRC2 (polycomb repressive complex 2) by targeting its free subunits for Ub-mediated proteasomal degradation and it can be involved in protein ubiquitination in the brain and is a suitable candidate gene for MRX(PMID:12036302). The Praja1 protein is unlikely to be a membrane receptor, since it lacks a hydrophobic transmembrane domain(PMID:9393880). It has 3 isoforms produced by alternative splicing with the molecular weight of 71 kDa, 50 kDa and 65 kDa.