PKC Delta Monoclonal antibody

• 

  WB analysis of HeLa using 67113-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67113-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67113-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67113-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67113-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67113-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 67113-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

PKC Delta Monoclonal Antibody for WB, ELISA

Cat No. 67113-1-Ig

Clone No.2B3E3

Publications(2)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

MAY1, PKCD, PRKCD, Protein kinase C delta type, protein kinase C, delta

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, A431 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

67113-1-Ig targets PKC Delta in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5631

  Product name: Recombinant human PRKCD protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 326-676 aa of BC043350

  Sequence: DMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED

• Full Name: protein kinase C, delta
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 78-80 kDa
• GenBank Accession Number: BC043350
• Gene Symbol: PKC Delta
• Gene ID (NCBI): 5580
• RRID: AB_2882417
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q05655
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IF	Int Immunopharmacol; Osthole alleviates neuropathic pain by suppressing astrocytes activation and associated inflammatory responses via the PKCδ/TRPV4 signaling pathway; Authors - Xiaoqian Xu
mouse	WB	Cell Death Differ; The PM20D1-NADA pathway protects against Parkinson's disease; Authors - Yunying Yang