PKIG Polyclonal antibody

PKIG Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21371-1-AP

Synonyms

PKI gamma, PKIG

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Validation Data Gallery

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  WB analysis of mouse heart using 21371-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21371-1-AP (PKIG antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 21371-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21371-1-AP (PKIG antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 21371-1-AP

  Immunofluorescent analysis of Hela cells, using PKIG antibody 21371-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 21371-1-AP is PKIG Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PKIG fusion protein Ag15878
• Full Name: protein kinase (cAMP-dependent, catalytic) inhibitor gamma
• Calculated Molecular Weight: 76 aa, 8 kDa
• Observed Molecular Weight: 5-8 kDa
• GenBank Accession Number: BC104257
• Gene Symbol: PKIG
• Gene ID (NCBI): 11142
• RRID: AB_10858922
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Biol Chem; cAMP-Dependent Protein Kinase (PKA) Signaling is Impaired in the Diabetic Heart.; Authors - Lee B Bockus