PKLR Polyclonal antibody

PKLR Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 17580-1-AP

Synonyms

PK1, PKL, PKLR, PKR, PKRL, Pyruvate kinase 1, Pyruvate kinase isozymes R/L, pyruvate kinase, liver and RBC, R type/L type pyruvate kinase, Red cell/liver pyruvate kinase, RPK



Tested Applications

Positive WB detected inK-562 cells, mouse kidney tissue, mouse liver tissue, NIH/3T3 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17580-1-AP targets PKLR in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKLR fusion protein Ag11096
Full Name pyruvate kinase, liver and RBC
Calculated molecular weight 574 aa, 62 kDa, 585 aa, 63 kDa
Observed molecular weight 58-62 kDa
GenBank accession numberBC025737
Gene symbol PKLR
Gene ID (NCBI) 5313
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PKLR antibody 17580-1-APDownload protocol
IHC protocol for PKLR antibody 17580-1-APDownload protocol
IP protocol for PKLR antibody 17580-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols