PKMYT1 Polyclonal antibody

PKMYT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55098-1-AP

Synonyms

MYT1, Myt1 kinase, PKMYT1

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Validation Data Gallery

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  WB analysis of mouse brain using 55098-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55098-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 55098-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55098-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 55098-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 55098-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, A375 cells, HeLa cells, HepG2 cells, HL-60 cells, mouse skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

55098-1-AP targets PKMYT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein kinase, membrane associated tyrosine/threonine 1
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: NM_004203
• Gene Symbol: PKMYT1
• Gene ID (NCBI): 9088
• RRID: AB_10888636
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase, membrane associated tyrosine/threonine (PKMYT1) is a member of the Wee family of protein kinases. PKMYT1 acts as a negative regulator of the cell cycle by inactivating the CDK1-cyclinB complex through phosphorylation of Tyr14/Tyr15 and preventing cell cycle entering into mitosis at the G2/M transition. In mammalian somatic cells, PKMYT1 affects the assembly of the Golgi apparatus and endoplasmic reticulum during telophase of mitosis by inhibiting cyclin activities. Abnormal expression of PKMYT1 is closely associated with multiple types of cancers. PKMYT1 protein was mainly localized in the cytoplasm. (PMID: 31695486, PMID: 32801781)

Publications

Species	Application	Title
human	WB	Thorac Cancer; Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3; Authors - Dong Zhou