PKP4 Polyclonal antibody

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• PKP4 Polyclonal antibody (14062-1-AP)

PKP4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14062-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

plakophilin 4, Plakophilin-4

• 

  WB analysis of A431 using 14062-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14062-1-AP (PKP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 14062-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14062-1-AP (PKP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse stomach using 14062-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14062-1-AP (PKP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat salivary gland using 14062-1-AP

  Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 14062-1-AP (PKP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat stomach using 14062-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 14062-1-AP (PKP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells
• Positive IHC detected in: rat salivary gland tissue, rat stomach tissue, mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14062-1-AP targets PKP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5113

  Product name: Recombinant human PKP4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 800-1149 aa of BC050308

  Sequence: DQWDGVGPIPGLSKSPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGKYAMRDLVNRLPGGNGPSVLSDETMAAICCALHEVTSKNMENAKALADSGGIEKLVNITKGRGDRSSLKVVKAAAQVLNTLWQYRDLRSIYKKDGWNQNHFITPVSTLERDRFKSHPSLSTTNQQMSPIIQSGSSKPSPIYISSYSSPAREQNRRLQHQQLYYSQDDSNRKNFDAYRLYLQSPHSYEDPYFDDRVHFPASTDYSTQYGLKSTTNYVDFYSTKRPSYRAEQYPGSPDSWV

• Full Name: plakophilin 4
• Calculated Molecular Weight: 132 kDa
• Observed Molecular Weight: 125-140 kDa
• GenBank Accession Number: BC050308
• Gene Symbol: PKP4
• Gene ID (NCBI): 8502
• RRID: AB_10640532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99569
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The PKP4 Polyclonal antibody (Cat# 14062-1-AP) has 1 total citation. It appears in Biochemical and Biophysical Research Communications (IF 2.5), in a study investigating how hydrogen sulfide improves keratinocyte migration and diabetic skin wound healing via plakophilin 4. The research field encompasses skin biology, wound healing, and diabetic complications. The antibody was used in Western Blot with human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Biochem Biophys Res Commun; Hydrogen sulfide improves keratinocytes migration and diabetic skin wound healing via plakophilin 4.; Authors - Bishan Zhang ; KO Validated