PLA2G3 Polyclonal antibody, PBS Only

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• PLA2G3 Polyclonal antibody, PBS Only (17646-1-PBS)

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  WB analysis using 17646-1-AP (same clone as 17646-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17646-1-AP (PLA2G3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17646-1-PBS in a different storage buffer formulation.

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  IP experiment of human milk using 17646-1-AP (same clone as 17646-1-PBS)

  IP result of anti-PLA2G3 (IP:17646-1-AP, 4ug; Detection:17646-1-AP 1:500) with human milk lysate 3600 ug. This data was developed using the same antibody clone with 17646-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 17646-1-AP (same clone as 17646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17646-1-AP (PLA2G3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17646-1-PBS in a different storage buffer formulation.

PLA2G3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 17646-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

EC:3.1.1.4, GIII sPLA2, Phosphatidylcholine 2-acylhydrolase 3, phospholipase A2, group III, sPLA2 III

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Product Information

17646-1-PBS targets PLA2G3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11845

  Product name: Recombinant human PLA2G3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 160-509 aa of BC025316

  Sequence: GVGDSAGNSSELGVFQGPDLCCREHDRCPQNISPLQYNYGIRNYRFHTISHCDCDTRFQQCLQNQHDSISDIVGVAFFNVLEIPCFVLEEQEACVAWYWWGGCRMYGTVPLARLQPRTFYNASWSSRATSPTPSSRSPAPPKPRQKQHLRKGPPHQKGSKRPSKANTTALQDPMVSPRLDVAPTGLQGPQGGLKPQGARWVCRSFRRHLDQCEHQIGPREIEFQLLNSAQEPLFHCNCTRRLARFLRLHSPPEVTNMLWELLGTTCFKLAPPLDCVEGKNCSRDPRAIRVSARHLRRLQQRRHQLQDKGTDERQPWPSEPLRGPMSFYNQCLQLTQAARRPDRQQKSWSQ

• Full Name: phospholipase A2, group III
• Calculated Molecular Weight: 509 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC025316
• Gene Symbol: PLA2G3
• Gene ID (NCBI): 50487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NZ20
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The PLA2G3 protein (full name: Phospholipase A2 Group III) is a member of the secreted phospholipase A2 (sPLA2) family. It is a calcium-dependent secretory protein whose primary biochemical function is to catalyze the hydrolysis of the sn-2 position of phospholipids, releasing arachidonic acid and lysophospholipids. It is an enzyme involved in the hydrolysis of membrane phospholipids and lipid metabolism, playing important roles in the development and progression of innate immunity, inflammatory diseases (such as asthma and psoriasis), atherosclerosis, and certain cancers (such as colorectal cancer and ovarian cancer).