Tested Applications
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13345-1-AP targets PLA2G4D in IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4178 Product name: Recombinant human PLA2G4D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC034571 Sequence: MGRLMRRIPEPRICFLEAIWSNIFSLNLLDAWYDLTSSGESWKQHIKDKTRSLEKEPLTTSGTSSRLEASWLQPGTALAQAFKGFLTGRPLHQRSPNFLQGLQLHQDYCSHKDFSTWADYQLDSMPSQLTPKEPRLCLVDAAYFINTSSPSMFRPGRRLDLILSFDYSLSAPFEVPWSPQGNPSAQPGQAPEASSRATEPLPHTARVPKGRRGVRP Predict reactive species |
| Full Name | phospholipase A2, group IVD (cytosolic) |
| Calculated Molecular Weight | 818 aa, 92 kDa |
| Observed Molecular Weight | 92 kDa |
| GenBank Accession Number | BC034571 |
| Gene Symbol | PLA2G4D |
| Gene ID (NCBI) | 283748 |
| RRID | AB_2164607 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86XP0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PLA2G4D(Cytosolic phospholipase A2 delta) belongs to the phospholipase A2 enzyme family,catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases(PMID:14709560).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PLA2G4D antibody 13345-1-AP | Download protocol |
| WB protocol for PLA2G4D antibody 13345-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PLA2G4D Polyclonal antibody (Cat# 13345-1-AP) has 1 citation to date. It was cited in Phytomedicine (IF: 11.3) in a study investigating multi-target mechanisms of Bojungikki-tang in psoriasis-like dermatitis using integrative network and transcriptomic analysis. The antibody was applied in IHC on mouse samples, aligning with its validated reactivity for human and mouse. The research field encompasses inflammatory skin diseases and traditional medicine pharmacology.
| Species | Application | Title |
|---|---|---|
Phytomedicine Multi-target mechanisms of Bojungikki-tang in psoriasis-like dermatitis: Integrative network and transcriptomic analysis. |
