PLA2G4D Polyclonal antibody

PLA2G4D Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 13345-1-AP

Synonyms

Cpla2 delta, cPLA2delta, Phospholipase A2 group IVD, PLA2G4D

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Validation Data Gallery

• 

  WB analysis of mouse brain using 13345-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13345-1-AP (PLA2G4D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 13345-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13345-1-AP (PLA2G4D Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 13345-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 13345-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 13345-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 13345-1-AP (PLA2G4D antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 13345-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 13345-1-AP (PLA2G4D antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin cancer using 13345-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 13345-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin cancer using 13345-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, Transfected HEK-293 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13345-1-AP targets PLA2G4D in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLA2G4D fusion protein Ag4178
• Full Name: phospholipase A2, group IVD (cytosolic)
• Calculated Molecular Weight: 818 aa, 92 kDa
• Observed Molecular Weight: 92 kDa
• GenBank Accession Number: BC034571
• Gene Symbol: PLA2G4D
• Gene ID (NCBI): 283748
• RRID: AB_2164607
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLA2G4D(Cytosolic phospholipase A2 delta) belongs to the phospholipase A2 enzyme family,catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases(PMID:14709560).