PLA2G4E Polyclonal antibody

PLA2G4E Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 29990-1-AP

Synonyms

FLJ45651, phospholipase A2, group IVE, PLA2G4E, PLA2G4E Ens

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Validation Data Gallery

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  WB analysis using 29990-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29990-1-AP (PLA2G4E antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 29990-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLA2G4E antibody (29990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, K-562 cells, NIH/3T3 cells
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29990-1-AP targets PLA2G4E in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLA2G4E fusion protein Ag32314
• Full Name: phospholipase A2, group IVE
• Calculated Molecular Weight: 868 aa, 99 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC101584
• Gene Symbol: PLA2G4E
• Gene ID (NCBI): 123745
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLA2G4E (Phospholipase A2 group IVE), also known as cPLA2-epsilon. Calcium-dependent N-acyltransferase is involved in the biosynthesis of N-acyl ethanolamines (NAEs) in the brain (PMID:29447909). Transfers the sn-1 fatty acyl chain of phosphatidylcholine (fatty acyl donor) to the amine group of phosphatidylethanolamine (fatty acyl acceptor) to generate N-acyl phosphatidylethanolamine (NAPE). Similarly can use plasmenylethanolamine as a fatty acyl acceptor to form N-acyl plasmenylethanolamine (N-Acyl-PlsEt). Both NAPE and N-Acyl-PlsEt can serve as precursors of bioactive NAEs like N-arachidonoyl phosphatidylethanolamine also called anandamide (PMID:29447909; 30517655).