Tested Applications
| Positive WB detected in | RAW 264.7 cells, mouse spleen tissue, mouse liver tissue, RAW264.7 |
| Positive IHC detected in | human tonsillitis tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15526-1-AP targets PAFAH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7227 Product name: Recombinant human PLA2G7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 92-438 aa of BC038452 Sequence: RLDTLWIPNKEYFWGLSKFLGTHWLMGNILRLLFGSMTTPANWNSPLRPGEKYPLVVFSHGLGAFRTLYSAIGIDLASHGFIVAAVEHRDRSASATYYFKDQSAAEIGDKSWLYLRTLKQEEETHIRNEQVRQRAKECSQALSLILDIDHGKPVKNALDLKFDMEQLKDSIDREKIAVIGHSFGGATVIQTLSEDQRFRCGIALDAWMFPLGDEVYSRIPQPLFFINSEYFQYPANIIKMKKCYSPDKERKMITIRGSVHQNFADFTFATGKIIGHMLKLKGDIDSNAAIDLSNKASLAFLQKHLGLHKDFDQWDCLIEGDDENLIPGTNINTTNQHIMLQNSSGIE Predict reactive species |
| Full Name | phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 43-67 kDa |
| GenBank Accession Number | BC038452 |
| Gene Symbol | PLA2G7 |
| Gene ID (NCBI) | 7941 |
| RRID | AB_2878146 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13093 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PAFAH(platelet-activating factor acetylhydrolase, plasma) is also named as PLA2G7(phospholipase A2, group VII), Lp-PLA2 (Lipoprotein-associated phospholipase A2), LDL-PLA2, LDL-PLA and belongs to the AB hydrolase superfamily and lipase family. It is an enzyme which catalyzes the hydrolysis of acetyl ester at the sn-2 position of PAF. The family of PAF-Ahs consists of two intracellular isoforms (Ib and II), and one secreted isoform (plasma). Plasma PAF-AH is a monomeric polypeptide with a molecular weight of 45 kDa and it can show broad bands with a molecular weight of 43-67 kDa that it can be extensively N-glycosylated(PMID:12547653). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PAFAD), susceptibility to asthma (ASTHMA), susceptibility to atopic hypersensitivity (ATOPY).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PAFAH antibody 15526-1-AP | Download protocol |
| IHC protocol for PAFAH antibody 15526-1-AP | Download protocol |
| WB protocol for PAFAH antibody 15526-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-PLA2G7 Affinity Purified Antibody (Cat# 15526-1-AP) has 17 citations spanning journals including Nature Metabolism, Nature Cell Biology, Nature Communications, Cell Death & Disease, Journal for ImmunoTherapy of Cancer, Cell Death Discovery, International Journal of Molecular Sciences, Scientific Reports, and ACS Chemical Biology. Research fields cover lipid metabolism, bone homeostasis, tumor immunology, inflammasome activation, ferroptosis, pulmonary fibrosis, and neuroinflammation.
| Species | Application | Title |
|---|---|---|
Nat Metab Elevated dietary ω-6 polyunsaturated fatty acids induce reversible peripheral nerve dysfunction that exacerbates comorbid pain conditions.
| ||
Nat Cell Biol Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation. | ||
J Extracell Vesicles Characterization of protein, long noncoding RNA and microRNA signatures in extracellular vesicles derived from resting and degranulated mast cells. | ||
Reviews
What customers say
One reviewer tested this PAFAH antibody for Western Blot on lysed mouse blood at dilutions ranging from 1:300 to 1:1000 but was unable to detect any target bands. Notably, loading controls from Proteintech on the same blot produced strong, clear bands, suggesting the issue was specific to this antibody rather than a general experimental problem. The reviewer rated it 3 out of 5 stars.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sathyanarayani (Verified Customer) (05-12-2023) | I used this PAFAH antibody at a range of different dilutions from 1:300 to 1:1000 but unfortunately I could not detect any bands from this antibody when I used lysed mouse blood. The loading controls which were also from Proteintech produced strong clear bands on the western blot.
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